The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus
Domingo Calap, María Luisa (Centre de Recerca en Agrigenòmica)
Chase, Ornela (Centre de Recerca en Agrigenòmica)
Estapé, Mariona (Centre de Recerca en Agrigenòmica)
Moreno, Ana Beatriz (Centre de Recerca en Agrigenòmica)
Lopez-Moya, Juan Jose (Centre de Recerca en Agrigenòmica)

Date:	2021
Abstract:	Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with significant increases in accumulation of the non-potyvirus partner, as in the case of melon plants infected by the potyvirus Watermelon mosaic virus (WMV) and the crinivirus Cucurbit yellow stunting disorder virus (CYSDV). To further explore the synergistic interaction between these two viruses, the activity of RNA silencing suppressors (RSSs) was addressed in transiently co-expressed combinations of heterologous viral products in Nicotiana benthamiana leaves. While the strong RSS activity of WMV Helper Component Proteinase (HCPro) was unaltered, including no evident additive effects observed when co-expressed with the weaker CYSDV P25, an unexpected negative effect of WMV P1 was found on the RSS activity of P25. Analysis of protein expression during the assays showed that the amount of P25 was not reduced when co-expressed with P1. The detrimental action of P1 on the activity of P25 was dose-dependent, and the subcellular localization of fluorescently labeled variants of P1 and P25 when transiently co-expressed showed coincidences both in nucleus and cytoplasm. Also, immunoprecipitation experiments showed interaction of tagged versions of the two proteins. This novel interaction, not previously described in other combinations of potyviruses and criniviruses, might play a role in modulating the complexities of the response to multiple viral infections in susceptible plants.
Grants:	Agencia Estatal de Investigación AGL2016-75529-R Ministerio de Ciencia e Innovación PID2019-105692RB-100 Ministerio de Economía y Competitividad BES-2014-068970 Agència de Gestió d'Ajuts Universitaris i de Recerca 2018/FI_B-00329 European Commission 657527 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Ciencia e Innovación CEX2019-000902-S
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	RNA silencing suppression ; Watermelon mosaic potyvirus ; Cucurbit yellow stunting disease crinivirus ; Plant virus mixed infection ; Virus pathogenesis in plants
Published in:	Frontiers in microbiology, Vol. 12 (March 2021) , art. 645530, ISSN 1664-302X

DOI: 10.3389/fmicb.2021.645530
PMID: 33828542

13 p, 2.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles