Argonaute 2 Controls Antiviral Activity against Sweet Potato Mild Mottle Virus in Nicotiana benthamiana
Kenesi, Erzsébet (Institute of Plant Biology)
Lopez-Moya, Juan Jose (Centre de Recerca en Agrigenòmica)
Orosz, László (University of Szeged. Department of Medical Microbiology and Immunobiology)
Burgyán, József (Agricultural Biotechnology Institute)
Lakatos, Lóránt (Institute of Plant Biology)

Date:	2021
Abstract:	RNA silencing is a sequence specific post-transcriptional mechanism regulating important biological processes including antiviral defense in plants. Argonaute (AGO) proteins, the catalytic subunits of the silencing complexes, are loaded with small RNAs to execute the sequence specific RNA cleavage or translational inhibition. Plants encode several AGO proteins and a few of them, especially AGO1 and AGO2, have been shown to be required for antiviral silencing. Previously, we have shown that the P1 protein of the sweet potato mild mottle virus (SPMMV) suppresses the primary RNA silencing response by inhibiting AGO1. To analyze the role of AGO2 in antiviral defense against the SPMMV, we performed a comparative study using a wild type and ago2 −/− mutant Nicotiana benthamiana. Here we show that the AGO2 of N. benthamiana attenuates the symptoms of SPMMV infection. Upon SPMMV infection the levels of AGO2 mRNA and protein are greatly increased. Moreover, we found that AGO2 proteins are loaded with SPMMV derived viral small RNAs as well as with miRNAs. Our results indicate that AGO2 protein takes over the place of AGO1 to confer antiviral silencing. Finally, we provide a plausible explanation for the AGO2 mediated recovery of an SPMMV-infected sweet potato.
Grants:	Ministerio de Ciencia e Innovación SEV-2015-0533 Agencia Estatal de Investigación CEX2019-000902-S Ministerio de Ciencia e Innovación PID2019-105692RB-100
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	RNA silencing ; AGO1 ; AGO2 ; Sweet potato mild mottle virus
Published in:	Plants, Vol. 10, Issue 5 (April 2021) , art. 867, ISSN 2223-7747

DOI: 10.3390/plants10050867
PMID: 33925878

10 p, 13.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles