A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
Jacobs-Cachá, Conxita (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Puig-Gay, Natalia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Vergara, Ander (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gabaldon, Maria-Alejandra (Hospital Universitari Vall d'Hebron)
Sellarés, Joana (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Villena-Ortiz, Yolanda (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Agraz Pamplona, Irene (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Moreso, Francesc (Universitat Autònoma de Barcelona. Departament de Medicina)
Soler, María José (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Seron, Daniel (Universitat Autònoma de Barcelona. Departament de Medicina)
López-Hellín, Joan (Hospital Universitari Vall d'Hebron. Institut de Recerca)

Fecha:	2021
Resumen:	A major complication of primary focal segmental glomerulosclerosis (FSGS) is its recurrence after kidney transplantation that happens in 30 to 40% of the patients. The diagnosis of these relapses is not always easy as the histological lesions are not highly specific and appear after the proteinuria increase. Currently, there are no accurate biomarkers to detect FSGS recurrence. Our group identified a modified form of Apolipoprotein A-I (ApoA-I), named ApoA-Ib, specifically present in the urine of recurrent FSGS patients after kidney transplantation. Aberrant forms of ApoA-I have also been described in the urine of native primary FSGS patients; this feature has been associated with prominent staining of ApoA-I at the apical membrane of the tubular cells. In this study, we aim to analyze the ApoA-I distribution in kidney allograft biopsies of recurrent FSGS patients. We detected ApoA-I by immunohistochemistry in kidney allograft biopsies of patients with FSGS relapse after kidney transplantation and in kidney allograft biopsies of patients with a disease different from FSGS in the native kidney (non-FSGS). In recurrent FSGS patients, ApoA-I was prominently localized at the brush border of the tubular cells, while in the non-FSGS patients, ApoA-I was found along the cytoplasm of the tubular cells. The localization of ApoA-I at the brush border of the tubular cells is a specific feature of primary FSGS in relapse. This suggests that ApoA-I staining in kidney biopsies, coupled with ApoA-Ib measurement in urine, could be used as a diagnostic tool of primary FSGS relapse after kidney transplantation due to its highly specific tubular distribution.
Ayudas:	Instituto de Salud Carlos III PI14/0027 Instituto de Salud Carlos III PI17/00257 Instituto de Salud Carlos III PI18/01704 Instituto de Salud Carlos III PI18/01
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Focal segmental glomerulosclerosis ; FSGS ; Recurrence ; Apolipoprotein A-I ; Apolipoprotein A-Ib ; ApoA-I ; ApoA-Ib ; Biomarkers ; Glomerular disease ; Kidney transplantation
Publicado en:	Journal of clinical medicine, Vol. 10 (may 2021) , ISSN 2077-0383

DOI: 10.3390/jcm10102174
PMID: 34069888

9 p, 2.6 MB

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