Anti-Inflammatory Effect of an O -2-Substituted (1-3)-β-D-Glucan Produced by Pediococcus parvulus 2.6 in a Caco-2 PMA-THP-1 Co-Culture Model
Notararigo, Sara (Hospital Universitari Vall d'Hebron)
Varela, Encarnación (Vall d'Hebron Institut de Recerca (VHIR))
Otal, Anna (Vall d'Hebron Institut de Recerca (VHIR))
Antolín, María (Vall d'Hebron Institut de Recerca (VHIR))
Guarner, Francisco (Vall d'Hebron Institut de Recerca (VHIR))
López, Paloma (Margarita Salas Center for Biological Research)

Fecha:	2022
Resumen:	Bacterial β-glucans are exopolysaccharides (EPSs), which can protect bacteria or cooperate in biofilm formation or in bacterial cell adhesion. Pediococcus parvulus 2. 6 is a lactic acid bacterium that produces an O -2-substituted (1-3)-β-D-glucan. The structural similarity of this EPS to active compounds such as laminarin, together with its ability to modulate the immune system and to adhere in vitro to human enterocytes, led us to investigate, in comparison with laminarin, its potential as an immunomodulator of in vitro co-cultured Caco-2 and PMA-THP-1 cells. O -2-substituted (1-3)-β-D-glucan synthesized by the GTF glycosyl transferase of Pediococcus parvulus 2. 6 or that by Lactococcus lactis NZ9000[pGTF] were purified and used in this study. The XTT tests revealed that all β-glucans were non-toxic for both cell lines and activated PMA-THP-1 cells' metabolisms. The O -2-substituted (1-3)-β-D-glucan modulated production and expression of IL-8 and the IL-10 in Caco-2 and PMA-THP-1 cells. Laminarin also modulated cytokine production by diminishing TNF-α in Caco-2 cells and IL-8 in PMA-THP-1. All these features could be considered with the aim to produce function foods, supplemented with laminarin or with another novel β-glucan-producing strain, in order to ameliorate an individual's immune system response toward pathogens or to control mild side effects in remission patients affected by inflammatory bowel diseases.
Ayudas:	Ministerio de Economía, Industria y Competitividad PI12/0026
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	(1-3)-β-D-glucan ; Bacterial exopolysaccharides ; Immunomodulators ; Pediococcus parvulus ; Post-biotic ; Anti-inflammatory
Publicado en:	International journal of molecular sciences, Vol. 23 (january 2022) , ISSN 1422-0067

DOI: 10.3390/ijms23031527
PMID: 35163449

20 p, 1.4 MB

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