On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies : Alkali Thermochemical Treatment and RGD Peptide Coating
Rappe, Katrin Steffanie (Universitat Autònoma de Barcelona. Departament de Cirurgia)
Ortiz-Hernandez, Monica (Institut de Recerca Sant Joan de Déu)
Punset, Miquel (Universitat Politècnica de Catalunya)
Molmeneu, Meritxell (Institut de Recerca Sant Joan de Déu)
Barba, Albert (Universitat Autònoma de Barcelona. Departament de Cirurgia)
Mas-Moruno, Carles (Institut de Recerca Sant Joan de Déu)
Guillem-Marti, Jordi (Institut de Recerca Sant Joan de Déu)
Caparrós, Cristina (Universitat Politècnica de Catalunya. Research Centre for Biomedical Engineering)
Rupérez, Elisa (Institut de Recerca Sant Joan de Déu)
Calero, José (AMES GROUP)
Manzanares, María-Cristina (Universitat de Barcelona. Departament de Patologia i Terapèutica Experimental)
Gil, Javier (Universitat Internacional de Catalunya. Bioengineering Institute of Technology)
Franch, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2022
Resum:	A lack of primary stability and osteointegration in metallic implants may result in implant loosening and failure. Adding porosity to metallic implants reduces the stress shielding effect and improves implant performance, allowing the surrounding bone tissue to grow into the scaffold. However, a bioactive surface is needed to stimulate implant osteointegration and improve mechanical stability. In this study, porous titanium implants were produced via powder sintering to create different porous diameters and open interconnectivity. Two strategies were used to generate a bioactive surface on the metallic foams: (1) an inorganic alkali thermochemical treatment, (2) grafting a cell adhesive tripeptide (RGD). RGD peptides exhibit an affinity for integrins expressed by osteoblasts, and have been reported to improve osteoblast adhesion, whereas the thermochemical treatment is known to improve titanium implant osseointegration upon implantation. Bioactivated scaffolds and control samples were implanted into the tibiae of rabbits to analyze the effect of these two strategies in vivo regarding bone tissue regeneration through interconnected porosity. Histomorphometric evaluation was performed at 4 and 12 weeks after implantation. Bone-to-implant contact (BIC) and bone in-growth and on-growth were evaluated in different regions of interest (ROIs) inside and outside the implant. The results of this study show that after a long-term postoperative period, the RGD-coated samples presented higher quantification values of quantified newly formed bone tissue in the implant's outer area. However, the total analyzed bone in-growth was observed to be slightly greater in the scaffolds treated with alkali thermochemical treatment. These results suggest that both strategies contribute to enhancing porous metallic implant stability and osteointegration, and a combination of both strategies might be worth pursuing.
Ajuts:	Agencia Estatal de Investigación RTI2018-098075-B-C21 Agencia Estatal de Investigación RTI2018-098075-BC22 Agencia Estatal de Investigación PID2020-114019RB-I00/AEI/10.13039/501100011033
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Titanium foams ; Osseointegration ; Thermochemical treatment ; RGD peptide ; In vivo implantation ; Histomorphometric evaluation ; Bone on-growth ; Bone in-growth
Publicat a:	International journal of molecular sciences, Vol. 23 (february 2022) , ISSN 1422-0067

DOI: 10.3390/ijms23031750
PMID: 35163682

26 p, 49.3 MB

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