A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
Tang, Xiaoqian (Université de Perpignan Via Domitia)
Zhang, Qi (Ministry of Agriculture and Rural Affairs. Quality Inspection and Test Center for Oilseeds Products)
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química)
Zhang, Zhaowei (Ministry of Agriculture and Rural Affairs. Laboratory of Risk Assessment for Oilseeds Products)
Marty, Jean-Louis (Sensbiotech)
Catanante, Gaëlle (Université de Perpignan Via Domitia)

Data:	2022
Resum:	Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2. 7~139. 8 ng/mL in the buffer and 7. 07~359. 5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B (AFB), citrinin or tenuazonic acid (TEA). The limit of detection was 0. 19 ng/mL in the buffer and 0. 36 ng/mL in the real sample. The recoveries were 83. 3% to 107. 1%, with a satisfactory RSD value from 6. 5% to 7. 5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice.
Ajuts:	Agencia Estatal de Investigación PID2019-106625RB-I00
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Patulin ; Aptamer ; Lateral flow assay ; On-site ; Apple juice
Publicat a:	Biosensors, Vol. 12, Issue 2 (January 2022) , art. 59, ISSN 2079-6374

DOI: 10.3390/bios12020059
PMID: 35200320

12 p, 1.0 MB

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