Chronic Glaucoma Using Biodegradable Microspheres to Induce Intraocular Pressure Elevation. Six-Month Follow-Up
Rodrigo Sanjuan, María Jesús (Universidad de Zaragoza. Miguel Servet Ophthalmology Research Group)
Garcia-Herranz, David (Hospital Clínico San Carlos (Madrid))
Subías, Manuel (Universidad de Zaragoza. Miguel Servet Ophthalmology Research Group)
Martínez-Rincón, Teresa (Universidad de Zaragoza. Miguel Servet Ophthalmology Research Group)
Mendez-Martínez, Silvia (Universidad de Zaragoza. Miguel Servet Ophthalmology Research Group)
Bravo-Osuna, Irene (Hospital Clínico San Carlos (Madrid))
Carretero i Romay, Ana (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
Ruberte, Jesús (Universitat Autònoma de Barcelona. Centre de Biotecnologia Animal i de Teràpia Gènica (CBATEG))
García-Feijoo, Julián (Hospital Clínico San Carlos (Madrid))
Pablo, Luis E (Universidad de Zaragoza. Miguel Servet Ophthalmology Research Group)
Herrero-Vanrell, Rocío (Hospital Clínico San Carlos (Madrid))
Garcia-Martin, Elena (Universidad de Zaragoza. Miguel Servet Ophthalmology Research Group)

Date:	2021
Abstract:	Background: To compare two prolonged animal models of glaucoma over 24 weeks of follow-up. A novel pre-trabecular model of chronic glaucoma was achieved by injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (10-20 µm) (Ms20/10) into the ocular anterior chamber to progressively increase ocular hypertension (OHT). Methods: Rat right eyes were injected to induce OHT: 50% received a suspension of Ms20/10 in the anterior chamber at 0, 2, 4, 8, 12, 16 and 20 weeks, and the other 50% received a sclerosing episcleral vein injection biweekly (EPIm). Ophthalmological clinical signs, intraocular pressure (IOP), neuroretinal functionality measured by electroretinography (ERG), and structural analysis of the retina, retinal nerve fiber layer (RNFL) and ganglion cell layer (GCL) protocols using optical coherence tomography (OCT) and histological exams were performed. Results: Both models showed progressive neuroretinal degeneration (p < 0. 05), and contralateral eye affectation. The Ms20/10 model showed a more progressive increase in IOP and better preservation of ocular surface. Although no statistical differences were found between models, the EPIm showed a tendency to produce thicker retinal and thinner GCL thicknesses, slower latency and smaller amplitude as measured using ERG, and more aggressive disturbances in retinal histology. In both models, while the GCL showed the greatest percentage loss of thickness, the RNFL showed the greatest and earliest rate of thickness loss. Conclusions: The intracameral model with biodegradable microspheres resulted more like the conditions observed in humans. It was obtained by a less-aggressive mechanism, which allows for adequate study of the pathology over longer periods.
Grants:	Instituto de Salud Carlos III PI20/00437 Instituto de Salud Carlos III PI17/01726 Instituto de Salud Carlos III PI17/01946 Agencia Estatal de Investigación MAT2017-83858-C2-1 Agencia Estatal de Investigación MAT2017-83858-C2-2 Instituto de Salud Carlos III RD16/0008/0004 Instituto de Salud Carlos III RD16/0008/0009 Instituto de Salud Carlos III RD16/0008/029
Note:	Altres ajuts: Rio Hortega Research Grant M17/00213, Research Group UCM 920415, UCM-Santander fellowship (CT17/17-CT17-18).
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Glaucoma ; Animal model ; Microspheres ; Neurodegeneration
Published in:	Biomedicines, Vol. 9 Núm. 6 (june 2021) , ISSN 2227-9059

DOI: 10.3390/biomedicines9060682
PMID: 34208744

26 p, 7.0 MB

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