Exploring the evolution and epidemiology of European CC1-MRSA-IV : tracking a multidrug-resistant community-associated meticillin-resistant Staphylococcus aureus clone
Earls, Megan R. (University of Dublin)
Steinig, Eike J. (Australian Institute of Tropical Health and Medicine)
Monecke, Stefan (InfectoGnostics Research Campus Jena)
Samaniego Castruita, José Alfredo (Copenhagen University Hospital)
Simbeck, Alexandra (University Hospital Regensburg)
Schneider-Brachert, Wulf (University Hospital Regensburg)
Vremerǎ, Teodora (University of Medicine and Pharmacy "Grigore T Popa")
Dorneanu, Olivia S. (University of Medicine and Pharmacy "Grigore T Popa")
Loncaric, Igor (University of Veterinary Medicine Vienna)
Bes, Michèle (Institut des Agents Infectieux)
Lacoma, Alicia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Prat i Aymerich, Cristina (University Medical Center Utrecht)
Wernery, Ulrich (Central Veterinary Research Laboratory)
Armengol-Porta, Marc (Dresden Municipal Hospital)
Blomfeldt, Anita (Akershus University Hospital)
Duchene, Sebastian (University of Melbourne)
Bartels, Mette D. (Copenhagen University Hospital)
Ehricht, Ralf (Friedrich-Schiller University)
Coleman, David C. (University of Dublin)
Universitat Autònoma de Barcelona

Fecha:	2021
Resumen:	This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern Romania; all of human origin) of the clone. Phylogenetic analysis was performed using a maximum-likelihood approach, a time-measured phylogeny was reconstructed using Bayesian analysis, and in silico microarray genotyping was performed to identify resistance, virulence-associated and SCC mec (staphylococcal cassette chromosome mec) genes. Isolates were typically sequence type 1 (190/204) and spa type t127 (183/204). Bayesian analysis indicated that European CC1-MRSA-IV emerged in approximately 1995 before undergoing rapid expansion in the late 1990s and 2000s, while spreading throughout Europe and into the Middle East. Phylogenetic analysis revealed an unstructured meticillin-resistant (MRSA) population, lacking significant geographical or temporal clusters. The MRSA were genotypically multidrug-resistant, consistently encoded seh, and intermittently (34/194) encoded an undisrupted hlb gene with concomitant absence of the lysogenic phage-encoded genes sak and scn. All MRSA also harboured a characteristic ~5350 nt insertion in SCC mec adjacent to orfX. Detailed demographic data from Denmark showed that there, the clone is typically (25/35) found in the community, and often (10/35) among individuals with links to South-Eastern Europe. This study elucidated the evolution and epidemiology of European CC1-MRSA-IV, which emerged from a meticillin-susceptible lineage prevalent in North-Eastern Romania before disseminating rapidly throughout Europe.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	CA-MRSA ; European CC1-MRSA-IV clone ; Evolution ; Epidemiology ; Phylogenomics ; Transmission
Publicado en:	Microbial Genomics, Vol. 7 (july 2021) , ISSN 2057-5858

DOI: 10.1099/mgen.0.000601
PMID: 34223815

9 p, 3.2 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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