Angiogenin in the Neurogenic Subventricular Zone After Stroke
Gabriel Salazar, Marina (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Lei, Ting (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Grayston, Alba (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Costa, Carme (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Medina-Gutiérrez, Esperanza (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Comabella, Manuel (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Montaner, Joan (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Rosell Novel, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Fecha:	2021
Resumen:	Ischemic stroke is a leading cause of death and disability worldwide with effective acute thrombolytic treatments. However, brain repair mechanisms related to spontaneous or rehabilitation-induced recovery are still under investigation, and little is known about the molecules involved. The present study examines the potential role of angiogenin (ANG), a known regulator of cell function and metabolism linked to neurological disorders, focusing in the neurogenic subventricular zone (SVZ). Angiogenin expression was examined in the mouse SVZ and in SVZ-derived neural stem cells (NSCs), which were exposed to exogenous ANG treatment during neurosphere formation as well as in other neuron-like cells (SH-SY5Y). Additionally, male C57Bl/6 mice underwent a distal permanent occlusion of the middle cerebral artery to study endogenous and exercise-induced expression of SVZ-ANG and neuroblast migration. Our results show that SVZ areas are rich in ANG, primarily expressed in DCX+ neuroblasts but not in nestin+NSCs. In vitro, treatment with ANG increased the number of SVZ-derived NSCs forming neurospheres but could not modify SH-SY5Y neurite differentiation. Finally, physical exercise rapidly increased the amount of endogenous ANG in the ipsilateral SVZ niche after ischemia, where DCX-migrating cells increased as part of the post-stroke neurogenesis process. Our findings position for the first time ANG in the SVZ during post-stroke recovery, which could be linked to neurogenesis.
Ayudas:	Instituto de Salud Carlos III PII15/00003 Instituto de Salud Carlos III FI17/00073 Ministerio de Economía y Competitividad PI16/00981 Instituto de Salud Carlos III PI19/00186 Instituto de Salud Carlos III RD16/0019/0021
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Stroke ; Angiogenin ; Neural stem/progenitor cells ; Neurogenesis ; Neurorepair ; Exercise
Publicado en:	Frontiers in neurology, Vol. 12 (june 2021) , ISSN 1664-2295

DOI: 10.3389/fneur.2021.662235
PMID: 34234733

10 p, 1.5 MB

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