Repeated sub-inhibitory doses of cassia essential oil do not increase the tolerance pattern in Listeria monocytogenes cells
Bermúdez-Capdevila, Maria (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Cervantes Huaman, Brayan Roger Hector (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Rodríguez-Jerez, José Juan (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Ripolles-Avila, Carolina (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Data:	2022
Resum:	Antimicrobial resistance in Listeria monocytogenes biofilms is considered a risk. When using disinfectants, the minimum inhibitory concentration (MIC) must be contemplated to avoid resistance. The objective of the present study was to determine the MIC of four essential oils in four L. monocytogenes strains. Once the MICs were obtained, the effect of subinhibitory doses of the most effective oil was determined. Strains were subsequently subjected to increasing doses of cassia and the MICs were determined again to evaluate differences. The effect of subinhibitory doses for biofilm formation was evaluated using quantitative and observational methodologies. Last, it was studied whether the strains were more sensitive to antibiotics after being in contact with the oil. After continuous exposure to 1/2 MIC, a decrease in the initial MIC (P = 0. 013) was observed, specifically for strains belonging to serotype 1/2a (P = 0. 041). In contrast, the formation of biofilms did not show differences between the control and exposed groups (P > 0. 05). The qualitative study showed that there were no differences in the structure of the biofilms before and after contact with cassia, except for the CECT 935 strain, indicating a straindependent trend. Moreover, species-dependent differences were observed in the conformation of the extracellular matrix.
Ajuts:	Ministerio de Ciencia, Innovación y Universidades RTI2018-098267-R-C32
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Listeria monocytogenes ; Biofilms ; Resistance ; Essential oils ; Food safety
Publicat a:	LWT, Vol. 165, 113681 (2022) , p. 1-9, ISSN 0023-6438

DOI: 10.1016/j.lwt.2022.113681

9 p, 5.5 MB

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