Polyphenols and IUGR Pregnancies: Intrauterine Growth Restriction and Hydroxytyrosol Affect the Development and Neurotransmitter Profile of the Hippocampus in a Pig Model
Yeste, Natalia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Gomez, Nestor (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Vázquez-Gómez, Marta (Universidad Complutense de Madrid)
García-Contreras, Consolación (INIA. Comparative Physiology Group)
Pumarola i Batlle, Martí (Universitat Autònoma de Barcelona. Unitat de Patologia Murina i Comparada)
Gonzalez-Bulnes, Antonio (Universidad CEU Cardenal Herrera. Departamento de Producción y Sanidad Animal)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Fecha:	2021
Resumen:	Intrauterine growth restriction (IUGR) refers to poor growth of a fetus during pregnancy due to deficient maternal nutrition or oxygen supply. Supplementation of a mother's diet with antioxidants, such as hydroxytyrosol (HTX), has been proposed to ameliorate the adverse phenotypes of IUGR. In the present study, sows were treated daily with or without 1. 5 mg of HTX per kilogram of feed from day 35 of pregnancy (at 30% of the total gestational period), and fetuses were sampled at day 100 of gestation. Fetuses were classified as normal body weight (NBW) or low body weight (LBW) as a consequence of IUGR, constituting four groups: NBW-Control, NBW-HTX, LBW-Control, and LBW-HTX. The brain was removed, and the hippocampus, amygdala, and prefrontal cortex were rapidly dissected. Neuronal markers were studied by immunohistochemistry, and a decrease in the number of mature neurons in the hippocampal Cornu Ammonis subfield 1 (CA1) and the Dentate Gyrus (DG) regions was observed in LBW fetuses together with a higher number of immature neurons and other alterations in neuronal morphology. Furthermore, IUGR conditions altered the neurotransmitter (NT) profile, since an increase in the serotonin (5-HT) pathway was observed in LBW fetuses. Supplementation with HTX was able to reverse the morphological and neurochemical changes, leading both characteristics to values similar to those of NBW fetuses.
Ayudas:	Ministerio de Economía y Competitividad AGL2016-79321-C2-1-R Ministerio de Ciencia e Innovación FPU17/01881 Ministerio de Ciencia e Innovación FPU14/01285 Ministerio de Economía y Competitividad AGL2013-48121-C3-R Ministerio de Economía y Competitividad AGL2015-68463-C2-2-P
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Hydroxytyrosol ; Neurotransmitters ; Intrauterine growth restriction ; Hippocampus ; Brain ; Pig
Publicado en:	Antioxidants, Vol. 10 (2021) , p. 1505, ISSN 2076-3921

DOI: 10.3390/antiox10101505
PMID: 34679640

15 p, 5.0 MB

Supplementary material 25.7 KB

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