Tryptophan front-face fluorescence and functional properties of whey : A preliminary study
Teng, Yu Ting (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Freire, Paulina (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Zamora, Anna (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Castillo, Manuel (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Fecha:	2022
Resumen:	This study investigated the potential of front-face fluorescence spectroscopy to predict the functional properties of whey. Whey has a commercial interest due to its excellent nutritional value and versatile functional properties. However, its attributes depend dramatically on heat treatment, which may alter its suitability for different food applications. Tryptophan front-face fluorescence of whey and its functional properties, i. e. , foaming, gel-forming, and emulsifying properties, were evaluated after milk heat treatment (at 80 °C with seven holding times) to detect correlations between tryptophan fluorescence and functional parameters and generate predictive models. Whey samples were obtained by isoelectric precipitation of caseins from reconstituted skim milk powder enriched with whey protein isolate. As expected, heat treatments induced an increase in whey protein denaturation, as well as a decrease in total whey protein concentration and tryptophan fluorescence intensity. Gel-forming and emulsifying properties of whey significantly correlated with the maximum intensity of tryptophan (P < 0. 001). Concerning foaming properties, only the foam stability index revealed a weak correlation with tryptophan maximum intensity (P < 0. 05). Consequently, models presented strong significances (P < 0. 001). Specifically, using only tryptophan fluorescence allowed successful prediction of emulsifying properties but was not enough for foaming and gel forming properties.
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Whey ; Front-face fluorescence ; Tryptophan fluorescence ; Heat treatment ; Functional properties
Publicado en:	LWT, Vol. 163 (June 2022) , art. 113589, ISSN 0023-6438

DOI: 10.1016/j.lwt.2022.113589

8 p, 781.7 KB

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