Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells
Astorga-Gamaza, Antonio (Vall d'Hebron Institut de Recerca (VHIR))
Vitali, Michele (Vall d'Hebron Institut de Recerca (VHIR))
Borrajo, Mireya L. (Vall d'Hebron Institut de Recerca (VHIR))
Suárez-López, Rosa (Universitat Autònoma de Barcelona. Departament de Química)
Jaime Cardiel, Carlos (Universitat Autònoma de Barcelona. Departament de Química)
Bastús, Neus G. (Institut Català de Nanociència i Nanotecnologia)
Serra-Peinado, Carla (Vall d'Hebron Institut de Recerca (VHIR))
Luque-Ballesteros, Laura (Vall d'Hebron Institut de Recerca (VHIR))
Blanch-Lombarte, Oscar (Institut Germans Trias i Pujol)
Prado, Julia G. (Institut Germans Trias i Pujol)
Lorente Guerrero, Juan (Hospital Universitari Vall d'Hebron)
Pumarola, Felix (Hospital Universitari Vall d'Hebron)
Pellicer, Marc (Hospital Universitari Vall d'Hebron)
Falcó, Vicenç (Vall d'Hebron Institut de Recerca (VHIR))
Genescà Ferrer, Meritxell (Vall d'Hebron Institut de Recerca (VHIR))
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Buzón, Maria José (Vall d'Hebron Institut de Recerca (VHIR))

Date:	2021
Abstract:	HIV represents a persistent infection which negatively alters the immune system. New tools to reinvigorate different immune cell populations to impact HIV are needed. Herein, a novel nanotool for the specific enhancement of the natural killer (NK) immune response towards HIV-infected T-cells has been developed. Bispecific Au nanoparticles (BiAb-AuNPs), dually conjugated with IgG anti-HIVgp120 and IgG anti-human CD16 antibodies, were generated by a new controlled, linker-free and cooperative conjugation method promoting the ordered distribution and segregation of antibodies in domains. The cooperatively-adsorbed antibodies fully retained the capabilities to recognize their cognate antigen and were able to significantly enhance cell-to-cell contact between HIV-expressing cells and NK cells. As a consequence, the BiAb-AuNPs triggered a potent cytotoxic response against HIV-infected cells in blood and human tonsil explants. Remarkably, the BiAb-AuNPs were able to significantly reduce latent HIV infection after viral reactivation in a primary cell model of HIV latency. This novel molecularly-targeted strategy using a bispecific nanotool to enhance the immune system represents a new approximation with potential applications beyond HIV.
Grants:	Agencia Estatal de Investigación RTI2018-101082-B-I00 Ministerio de Economía y Competitividad SAF2015-67334-R Instituto de Salud Carlos III PI17/01470 Ministerio de Economía y Competitividad PI14/01058 Ministerio de Economía y Competitividad PI14/00179 Instituto de Salud Carlos III CPII15-000014 Agencia Estatal de Investigación BES-2016-076382 Agència de Gestió d'Ajuts Universitaris i de Recerca FI-B-00582
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	HIV ; Bispecific nanoparticles ; NK cells ; Polarization
Published in:	Nano Today, Vol. 36 (Feb. 2021) , art. 101056, ISSN 1878-044X

DOI: 10.1016/j.nantod.2020.101056
PMID: 34394703

15 p, 4.8 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles