Experimental and field investigations of exposure, replication and transmission of SARS-CoV-2 in pigs in the Netherlands
Sikkema, Reina S. (Erasmus Medical Centre)
Tobias, Tijs (Utrecht University)
Oreshkova, Nadia (Wageningen Bioveterinary Research)
de Bruin, Erwin (Erasmus Medical Centre)
Okba, Nisreen M. A (Erasmus Medical Centre)
Chandler, Felicity (Erasmus Medical Centre)
Hulst, Marcel M. (Wageningen Bioveterinary Research)
Rodon, Jordi (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Houben, Manon (Royal GD Animal Health)
van Maanen, Kees (Royal GD Animal Health)
Bultman, Hans (Royal GD Animal Health)
Meester, Marina (Utrecht University)
Gerhards, Nora M. (Wageningen Bioveterinary Research)
Bouwknegt, Martijn (Vion Food Group)
Urlings, Bert (Vion Food Group)
Haagmans, Bart L (Erasmus Medical Centre)
Kluytmans, Jan (University Medical Center Utrecht, Utrecht University)
Geurtsvankessel, Corine (Erasmus Medical Centre)
van der Poel, Wim H.M. (Wageningen Bioveterinary Research)
Koopmans, Marion P.G. (Erasmus Medical Centre)
Stegeman, Arjan (Utrecht University)

Fecha:	2021
Resumen:	In order to assess the risk of SARS-CoV-2 infection, transmission and reservoir development in swine, we combined results of an experimental and two observational studies. First, intranasal and intratracheal challenge of eight pigs did not result in infection, based on clinical signs and PCR on swab and lung tissue samples. Two serum samples returned a low positive result in virus neutralization, in line with findings in other infection experiments in pigs. Next, a retrospective observational study was performed in the Netherlands in the spring of 2020. Serum samples (N =417) obtained at slaughter from 17 farms located in a region with a high human case incidence in the first wave of the pandemic. Samples were tested with protein micro array, plaque reduction neutralization test and receptor-binding-domain ELISA. None of the serum samples was positive in all three assays, although six samples from one farm returned a low positive result in PRNT (titers 40-80). Therefore we conclude that serological evidence for large scale transmission was not observed. Finally, an outbreak of respiratory disease in pigs on one farm, coinciding with recent exposure to SARS-CoV-2 infected animal caretakers, was investigated. Tonsil swabs and paired serum samples were tested. No evidence for infection with SARS-CoV-2 was found. In conclusion, Although in both the experimental and the observational study few samples returned low antibody titer results in PRNT infection with SARS-CoV-2 was not confirmed. It was concluded that sporadic infections in the field cannot be excluded, but large-scale SARS-CoV-2 transmission among pigs is unlikely.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Swine ; Coronavirus ; SARS-CoV-2 ; Antibody ; One Health
Publicado en:	Emerging microbes & infections, Vol. 11 (december 2021) , p. 91-94, ISSN 2222-1751

DOI: 10.1080/22221751.2021.2011625
PMID: 34839786

4 p, 2.5 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Artículos > Artículos de investigación
Artículos > Artículos publicados