Maternal Phylogenetic Relationships and Genetic Variation among Rare, Phenotypically Similar Donkey Breeds
Mazzatenta, Andrea (University G. D'Annunzio of Chieti-Pescara. Imaging and Clinical Sciences Department)
Vignoli, Massimo (University of Teramo. Faculty of Veterinary Medicine)
Caputo, Maurizio (University of Teramo. Faculty of Veterinary Medicine)
Vignola, Giorgio (University of Teramo. Faculty of Veterinary Medicine)
Tamburro, Roberto (University of Teramo. Faculty of Veterinary Medicine)
De Sanctis, Francesco (University of Verona. Section of Immunology, Department of Medicine)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Bucci, Roberta (University of Teramo. Faculty of Veterinary Medicine)
Robbe, Domenico (University of Teramo. Faculty of Veterinary Medicine)
Carluccio, Augusto (University of Teramo. Faculty of Veterinary Medicine)

Date:	2021
Abstract:	The mitochondrial DNA (mtDNA) D-loop of endangered and critically endangered breeds has been studied to identify maternal lineages, characterize genetic inheritance, reconstruct phylogenetic relations among breeds, and develop biodiversity conservation and breeding programs. The aim of the study was to determine the variability remaining and the phylogenetic relationship of Martina Franca (MF, with total population of 160 females and 36 males), Ragusano (RG, 344 females and 30 males), Pantesco (PT, 47 females and 15 males), and Catalonian (CT) donkeys by collecting genetic data from maternal lineages. Genetic material was collected from saliva, and a 350 bp fragment of D-loop mtDNA was amplified and sequenced. Sequences were aligned and evaluated using standard bioinformatics software. A total of 56 haplotypes including 33 polymorphic sites were found in 77 samples (27 MF, 22 RG, 8 PT, 19 CT, 1 crossbred). The breed nucleotide diversity value (π) for all the breeds was 0. 128 (MF: 0. 162, RG: 0. 132, PT: 0. 025, CT: 0. 038). Principal components analysis grouped most of the haplogroups into two different clusters, I (including all haplotypes from PT and CT, together with haplotypes from MF and RG) and II (including haplotypes from MF and RG only). In conclusion, we found that the primeval haplotypes, haplogroup variability, and a large number of maternal lineages were preserved in MF and RG; thus, these breeds play putative pivotal roles in the phyletic relationships of donkey breeds. Maternal inheritance is indispensable genetic information required to evaluate inheritance, variability, and breeding programs.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Genetics ; Mitochondrial DNA ; Donkey ; Martina Franca ; Ragusano ; Pantesco ; Catalonian
Published in:	Genes, Vol. 12 (july 2021) , ISSN 2073-4425

DOI: 10.3390/genes12081109
PMID: 34440283

14 p, 1.9 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles