Development and Evaluation of an Axiom TM 60K SNP Array for Almond (Prunus dulcis)
Duval, Henri (Institut National de la Recherche Agronomique (França))
Coindre, Eva (Institut National de la Recherche Agronomique (França))
Ramos Onsins, Sebastián Ernesto (Centre de Recerca en Agrigenòmica)
Alexiou, Konstantinos G. (Centre de Recerca en Agrigenòmica)
Rubio-Cabetas, Maria J. (Centro de Investigación y Tecnología Agroalimentaria de Aragón)
Martínez-García, Pedro J. (Campus Universitario de Espinardo)
Wirthensohn, Michelle (Waite Research Institute, University of Adelaide)
Dhingra, Amit (Washington State University)
Samarina, Anna (Thermo Fisher Scientific)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)

Fecha:	2023
Resumen:	A high-density single nucleotide polymorphism (SNP) array is essential to enable faster progress in plant breeding for new cultivar development. In this regard, we have developed an Axiom 60K almond SNP array by resequencing 81 almond accessions. For the validation of the array, a set of 210 accessions were genotyped and 82. 8% of the SNPs were classified in the best recommended SNPs. The rate of missing data was between 0. 4% and 2. 7% for the almond accessions and less than 15. 5% for the few peach and wild accessions, suggesting that this array can be used for peach and interspecific peach × almond genetic studies. The values of the two SNPs linked to the RMja (nematode resistance) and SK (bitterness) genes were consistent. We also genotyped 49 hybrids from an almond F2 progeny and could build a genetic map with a set of 1159 SNPs. Error rates, less than 1%, were evaluated by comparing replicates and by detection of departures from Mendelian inheritance in the F2 progeny. This almond array is commercially available and should be a cost-effective genotyping tool useful in the search for new genes and quantitative traits loci (QTL) involved in the control of agronomic traits.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Prunus dulcis ; Prunus persica ; SNP ; Axiom array ; Genotyping
Publicado en:	Plants, Vol. 12, Num.2 (January 2023) , art 242, ISSN 2223-7747

DOI: 10.3390/plants12020242
PMID: 36678957

12 p, 1.6 MB

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