Enzymatic Synthesis of Ascorbyl Palmitate in a Rotating Bed Reactor
Holtheuer, Jessica (Pontificia Universidad Católica de Valparaíso)
Tavernini, Luigi (Pontificia Universidad Católica de Valparaíso)
Bernal, Claudia (Universidad de La Serena)
Romero, Oscar (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Ottone, Carminna (Pontificia Universidad Católica de Valparaíso)
Wilson, Lorena (Pontificia Universidad Católica de Valparaíso)

Date:	2023
Abstract:	Ascorbyl palmitate, an ascorbic acid ester, is an important amphipathic antioxidant that has several applications in foods, pharmaceuticals, and cosmetics. The enzymatic synthesis of ascorbyl palmitate is very attractive, but few efforts have been made to address its process scale-up and implementation. This study aimed at evaluating the enzymatic synthesis of ascorbyl palmitate in a rotating basket reactor operated in sequential batches. Different commercial immobilized lipases were tested, and the most suitable reaction conditions were established. Among those lipases studied were Amano Lipase PS, Lipozyme ® TL IM, Lipozyme ® Novo 40086, Lipozyme ® RM IM and Lipozyme ® 435. Initially, the enzymes were screened based on previously defined synthesis conditions, showing clear differences in behavior. Lipozyme ® 435 proved to be the best catalyst, reaching the highest values of initial reaction rate and yield. Therefore, it was selected for the following studies. Among the solvents assayed, 2-methyl-2-butanol and acetone showed the highest yields, but the operational stability of the catalyst was better in 2-methyl-2-butanol. The tests in a basket reactor showed great potential for large-scale application. Yields remained over 80% after four sequential batches, and the basket allowed for easy catalyst recycling. The results obtained in basket reactor are certainly a contribution to the enzymatic synthesis of ascorbyl palmitate as a competitive alternative to chemical synthesis. This may inspire future cost-effectiveness studies of the process to assess its potential as a viable alternative to be implemented.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2019/BP-00180
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Rotating bed reactor ; Lipase ; Enzymatic synthesis ; Ascorbyl palmitate
Published in:	Molecules, Vol. 28, Num. 2 (January 2023) , art. 644, ISSN 1420-3049

DOI: 10.3390/molecules28020644
PMID: 36677702

12 p, 1.2 MB

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Articles > Research articles
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