Development of a hybrid bioinorganic nanobiocatalyst : Remarkable impact of the immobilization conditions on activity and stability of β-galactosidase
Tavernini, Luigi (Pontificia Universidad Católica de Valparaíso)
Romero, Oscar (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Aburto, Carla (Pontificia Universidad Católica de Valparaíso)
López-Gallego, Fernando (Ikerbasque. Basque Foundation for Science)
Illanes, Andrés (Pontificia Universidad Católica de Valparaíso)
Wilson, Lorena (Pontificia Universidad Católica de Valparaíso)

Date:	2021
Abstract:	Hybrid bioinorganic biocatalysts have received much attention due to their simple syn-thesis, high efficiency, and structural features that favor enzyme activity and stability. The present work introduces a biomineralization strategy for the formation of hybrid nanocrystals from β-ga-lactosidase. The effects of the immobilization conditions were studied, identifying the important effect of metal ions and pH on the immobilization yield and the recovered activity. For a deeper understanding of the biomineralization process, an in silico study was carried out to identify the ion binding sites at the different conditions. The selected β-galactosidase nanocrystals showed high specific activity (35,000 IU/g biocatalyst) and remarkable thermal stability with a half-life 11 times higher than the soluble enzyme. The nanobiocatalyst was successfully tested for the synthesis of galacto-oligosaccharides, achieving an outstanding performance, showing no signs of diffusional limitations. Thus, a new, simple, biocompatible and inexpensive nanobiocatalyst was produced with high enzyme recovery (82%), exhibiting high specific activity and high stability, with promis-ing industrial applications.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Molecules, Vol. 26, Issue 14 (July 2021) , art. 4152, ISSN 1420-3049

DOI: 10.3390/molecules26144152
PMID: 34299429

14 p, 2.4 MB

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