A rapid calprotectin test for the diagnosis of pleural effusion
Casado-Rey, P. (Hospital Álvaro Cunqueiro (Vigo))
Vázquez-Iglesias, L. (Instituto de Investigación Sanitaria Galicia Sur)
Botana-Rial, M. (Instituto de Investigación Sanitaria Galicia Sur)
Andrade-Olivié, M.A. (Hospital Álvaro Cunqueiro (Vigo))
Ferreiro-Fernández, L. (Instituto de Investigación Sanitaria de Santiago (IDIS))
José Capilla, E.S. (Clinical Chemistry Department. University Hospital Complex of Santiago. EOXI)
Núñez-Ares, Ana (Complejo Hospitalario Universitario de Albacete)
de Miguel, E.B. (Pulmonary Department. University Hospital Complex of León)
Pajares Ruiz, Virginia (Institut d'Investigació Biomèdica Sant Pau)
Fernández-Villar, A. (Instituto de Investigación Sanitaria Galicia Sur)

Fecha:	2021
Resumen:	In previous studies, measuring the levels of calprotectin in patients with pleural effusion (PE) was an exceptionally accurate way to predict malignancy. Here, we evaluated a rapid method for the measurement of calprotectin levels as a useful parameter in the diagnosis of malignant pleural effusion (MPE) in order to minimise invasive diagnostic tests. Calprotectin levels were measured with Quantum Blue sCAL (QBsCAL) and compared with the gold standard reference ELISA method. Calprotectin levels in patients with benign pleural effusion (BPE) were significantly higher (p < 0. 0001) than for MPE patients. We measured the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and positive and negative likelihood ratios (LRs) for a cut-off value of ≤ 14,150 ng/mL; the diagnostic accuracy was 64%. The odds ratio for PE calprotectin levels was 10. 938 (95% CI [4. 133 − 28. 947]). The diagnostic performance of calprotectin concentration was better for predicting MPE compared to other individual parameters. Comparison of two assays showed a slope of 1. 084, an intercept of 329. 7, and a Pearson correlation coefficient of 0. 798. The Bland-Altman test showed a positive bias for the QBsCAL method compared to ELISA fCAL. Clinical concordance between both these methods was 88. 5% with a Cohen kappa index of 0. 76 (95% CI [0. 68 − 0. 84]). We concluded that QBsCAL is a fast, reliable, and non-invasive diagnostic tool for diagnosing MPE and represents an alternative to ELISA that could be implemented in medical emergencies.
Ayudas:	Instituto de Salud Carlos III PI13/01538
Nota:	Altres ajuts: Xunta de Galicia (CN/2014); grant of societies AEER (2015); SOGAPAR (2014); Fundación Biomédica Galicia Sur, integrated research programs of SEPAR (PII Neumología Intervencionista 2015).
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Aged ; Clinical Decision-Making ; Diagnostic Techniques and Procedures ; Female ; Humans ; Leukocyte L1 Antigen Complex ; Logistic Models ; Male ; Middle Aged ; Multivariate Analysis ; Pleural Effusion ; Reproducibility of Results
Publicado en:	PloS one, Vol. 16 Núm. 6 June (june 2021) , p. e0252714, ISSN 1932-6203

DOI: 10.1371/journal.pone.0252714
PMID: 34111153

13 p, 865.1 KB

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