Antiplatelet activity of isorhamnetin via mitochondrial regulation
Rodríguez, L. (Thrombosis Research Center. Department of Clinical Biochemistry and Immunohaematology. Faculty of Health Sciences. Medical Technology School. Universidad de Talca)
Badimon, Lina (Institut d'Investigació Biomèdica Sant Pau)
Méndez, D. (Thrombosis Research Center. Department of Clinical Biochemistry and Immunohaematology. Faculty of Health Sciences. Medical Technology School. Universidad de Talca)
Padró, Teresa (Institut d'Investigació Biomèdica Sant Pau)
Vilahur, Gemma (Institut d'Investigació Biomèdica Sant Pau)
Peña, Esther (Institut d'Investigació Biomèdica Sant Pau)
Carrasco, B. (Centro de Estudios en Alimentos Procesados)
Vogel, H. (CENATIV. Departamento de Horticultura. Facultad de Ciencias Agrarias. Universidad de Talca)
Palomo, I. (Thrombosis Research Center. Department of Clinical Biochemistry and Immunohaematology. Faculty of Health Sciences. Medical Technology School. Universidad de Talca)
Fuentes, E. (Thrombosis Research Center. Department of Clinical Biochemistry and Immunohaematology. Faculty of Health Sciences. Medical Technology School. Universidad de Talca)

Fecha:	2021
Resumen:	With the diet, we ingest nutrients capable of modulating platelet function, which plays a crucial role in developing cardiovascular events, one of the leading causes of mortality worldwide. Studies that demonstrate the antiplatelet and antithrombotic potential of bioactive compounds are vital to maintaining good cardiovascular health. In this work, we evaluate the flavonol isorhamnetin's antiplatelet effect on human platelets, using collagen, thrombin receptor activator peptide 6 (TRAP-6), and phorbol myristate acetate (PMA) as agonists. Isorhamnetin induced a significant inhibition on collagen-and TRAP-6-induced platelet aggregation, with half-maximum inhibitory concentration (IC) values of 8. 1 ± 2. 6 and 16. 1 ± 11. 1 µM, respectively; while it did not show cytotoxic effect. Isorhamnetin reduced adenosine triphosphate levels (ATP) in platelets stimulated by collagen and TRAP-6. We also evidenced that isorhamnetin's antiplatelet activity was related to the inhibition of mitochondrial function without effect on reactive oxygen species (ROS) levels. Additionally, we investigated isorhamnetin's effect on thrombus formation in vitro under flow conditions on the damaged vessel wall. In this context, we demonstrate that isorhamnetin at 20 µM induced a significant inhibition on platelet deposition, confirming its antithrombotic effect. Our findings corroborate the antiplatelet and antithrombotic potential of isorhamnetin present in many foods of daily consumption.
Nota:	Altres ajuts: Centro de Estudios en Alimentos Procesados-CEAP, ANID (National Doctorate Scholarship-Folio: 21191143, ANID R19A10001), Programa Regional R20F0001, Talca, Chile; CiberCV; Instituto Carlos III; Fundación de Investigación Cardiovascular-Fundación Jesús Serra, Spain.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Isorhamnetin ; Flavonoids ; Antithrombotic ; Antiplatelet ; Foods
Publicado en:	Antioxidants, Vol. 10 Núm. 5 (may 2021) , p. 666, ISSN 2076-3921

DOI: 10.3390/antiox10050666
PMID: 33922903

12 p, 2.0 MB

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