Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
Zhao, Lei (Institut Català de Nanociència i Nanotecnologia)
Rosati, Giulio (Institut Català de Nanociència i Nanotecnologia)
Piper, Andrew (Institut Català de Nanociència i Nanotecnologia)
De Carvalho Castro Silva, Cecilia (Mackenzie Presbyterian University)
Hu, Liming (Institut Català de Nanociència i Nanotecnologia)
Yang, Qiuyue (Institut Català de Nanociència i Nanotecnologia)
Della Pelle, Flavio (University of Teramo)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data:	2023
Resum:	Graphene-based materials are of interest in electrochemical biosensing due to their unique properties, such as high surface areas, unique electrochemical properties, and biocompatibility. However, the scalable production of graphene electrodes remains a challenge; it is typically slow, expensive, and inefficient. Herein, we reported a simple, fast, and maskless method for large-scale, low-cost reduced graphene oxide electrode fabrication; using direct writing (laser scribing and inkjet printing) coupled with a stamp-transferring method. In this process, graphene oxide is simultaneously reduced and patterned with a laser, before being press-stamped onto polyester sheets. The transferred electrodes were characterized by SEM, XPS, Raman, and electrochemical methods. The biosensing utility of the electrodes was demonstrated by developing an electrochemical test for Escherichia coli. These biosensors exhibited a wide dynamic range (917-2. 1 × 10 7 CFU/mL) of low limits of detection (283 CFU/mL) using just 5 μL of sample. The test was also verified in spiked artificial urine, and the sensor was integrated into a portable wireless system driven and measured by a smartphone. This work demonstrates the potential to use these biosensors for real-world, point-of-care applications. Hypothetically, the devices are suitable for the detection of other pathogenic bacteria.
Ajuts:	Agencia Estatal de Investigación SEV-2017-0706 European Commission 825694
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Graphene electrodes ; Fabrication ; Biosensing ; Nanomaterials ; Bacteria detection
Publicat a:	ACS applied materials & interfaces, Vol. 15, Issue 7 (February 2023) , p. 9024-9033, ISSN 1944-8252

DOI: 10.1021/acsami.2c20859
PMID: 36786303

10 p, 4.6 MB

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