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| Página principal > Artículos > Artículos publicados > Measurement of Calprotectin (S100A8/A9) in the Saliva of Pigs : |
(Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab) (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab) (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab) (Universidad de Murcia. Departamento de Producción Animal) (University College Dublin. School of Veterinary Medicine) (University of Glasgow. School of Biodiversity) (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab) (Universidad de Murcia. Departamento de Producción Animal) (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
| Fecha: | 2023 |
| Resumen: | Calprotectin (CALP, S100A8/A9) is a calcium and zinc-binding protein involved in inflammation that has a wide range of proinflammatory functions, such as cytokine production and regulation of leukocyte adhesion, migration, and phagocytosis. The objective of this study was to validate a commercially available assay for the measurement of CALP in the saliva of pigs and study the variations of this analyte due to sepsis, non-septic inflammation, and stress. The assay showed adequate precision and accuracy for the measurements of CALP in the saliva of pigs. In addition, this protein showed significant increases in the saliva of pigs with sepsis as well as after a stressful situation in our experimental conditions, being the increase in the stress of lower magnitude than in sepsis. Based on these results, CALP can be measured in the saliva of pigs and could be a potential biomarker of health and welfare in this species. Calprotectin (CALP, S100A8/A9), also named myeloid-related protein 8/14, is a dimer complex of S100A8 and S100A9 that belongs to the S-100 protein family. It is involved in inflammation and has a wide range of proinflammatory functions, such as cytokine production and regulation of leukocyte adhesion, migration, and phagocytosis. In humans, CALP traditionally can be measured in faeces, serum, and saliva as a biomarker of inflammation and sepsis. The objective of this study was to validate an automated assay for CALP measurements in the saliva of pigs, having the advantage of the use of a non-invasive sample that is easy to collect. The assay was precise and accurate. CALP in saliva measured by this assay showed significant changes depending on the hour of the day. It also showed significant increases in the saliva of pigs after the administration of lipopolysaccharide (LPS), and showed a rise, although with increases of lower magnitude, after a stressful stimulus. Further studies should be made to gain knowledge about the possible practical applications of the measurements of CALP in the saliva of pigs as a biomarker to evaluate the animals' health and welfare. |
| Ayudas: | Ministerio de Ciencia e Innovación PID2019-105950RB-100 Ministerio de Ciencia e Innovación PCI2020-120712-2 Ministerio de Ciencia e Innovación FJC2021-047105-I Ministerio de Ciencia e Innovación RYC2021-033660-I |
| Nota: | Altres ajuts: Fundación Séneca, Región de Murcia 21293/FPI/19 |
| Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Lengua: | Anglès |
| Documento: | Article ; recerca ; Versió publicada |
| Materia: | S100A8/A9 ; Calprotectin ; Automated assay ; Pig ; Saliva ; Stress ; Sepsis |
| Publicado en: | Animals, Vol. 13 (march 2023) , ISSN 2076-2615 |
