Genomic analysis of Mycobacterium brumae sustains its nonpathogenic and immunogenic phenotype
Renau-Mínguez, Chantal (University of Valencia-FISABIO Joint Unit)
Herrero Abadía, Paula (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Ruiz-Rodriguez, Paula (University of Valencia-FISABIO Joint Unit)
Sentandreu, Vicente (University of Valencia)
Torrents, Eduard (Universitat de Barcelona)
Chiner-Oms, Álvaro (Instituto de Biomedicina de Valencia (IBV-CSIC))
Torres-Puente, Manuela (Instituto de Biomedicina de Valencia (IBV-CSIC))
Comas, I (Instituto de Biomedicina de Valencia (IBV-CSIC))
Julián Gómez, Esther (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Coscolla, Mireia (University of Valencia-FISABIO Joint Unit)

Fecha:	2023
Resumen:	Mycobacterium brumae is a rapid-growing, non-pathogenic Mycobacterium species, originally isolated from environmental and human samples in Barcelona, Spain. Mycobacterium brumae is not pathogenic and it's in vitro phenotype and immunogenic properties have been well characterized. However, the knowledge of its underlying genetic composition is still incomplete. In this study, we first describe the 4 Mb genome of the M. brumae type strain ATCC 51384 T assembling PacBio reads, and second, we assess the low intraspecies variability by comparing the type strain with Illumina reads from three additional strains. Mycobacterium brumae genome is composed of a circular chromosome with a high GC content of 69. 2% and containing 3,791 CDSs, 97 pseudogenes, one prophage and no CRISPR loci. Mycobacterium brumae has shown no pathogenic potential in in vivo experiments, and our genomic analysis confirms its phylogenetic position with other non-pathogenic and rapid growing mycobacteria. Accordingly, we determined the absence of virulence-related genes, such as ESX-1 locus and most PE/PPE genes, among others. Although the immunogenic potential of M. brumae was proved to be as high as Mycobacterium bovis BCG, the only mycobacteria licensed to treat cancer, the genomic content of M. tuberculosis T cell and B cell antigens in M. brumae genome is considerably lower than those antigens present in M. bovis BCG genome. Overall, this work provides relevant genomic data on one of the species of the mycobacterial genus with high therapeutic potential.
Ayudas:	Agencia Estatal de Investigación RTI2018-098777-B-I00 Agencia Estatal de Investigación RTI2018-098573-B-I00 Agencia Estatal de Investigación RTI2018-094399-A-I00 Agencia Estatal de Investigación PID2021-123443OB-I00 Agencia Estatal de Investigación PID2021-122331OB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1079 Ministerio de Economía y Competitividad RYC-2015-18213 Agencia Estatal de Investigación PRE2019-088141 Agencia Estatal de Investigación RED2018-102677-T
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Diversity ; Immunogenic ; Non-pathogenic ; Nontuberculous mycobacteria ; Therapeutic
Publicado en:	Frontiers in microbiology, Vol. 13 (January 2023) , art. 982679, ISSN 1664-302X

DOI: 10.3389/fmicb.2022.982679
PMID: 36687580

19 p, 9.2 MB

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