Profiling of Myositis Specific Antibodies and Composite Scores as an Aid in the Differential Diagnosis of Autoimmune Myopathies
Mahler, Michael (Inova Diagnostics (San Diego, Estats Units d'Amèrica))
Malyavantham, Kishore (Inova Diagnostics (San Diego, Estats Units d'Amèrica))
Seaman, Andrea (Inova Diagnostics (San Diego, Estats Units d'Amèrica))
Bentow, Chelsea (Inova Diagnostics (San Diego, Estats Units d'Amèrica))
Anunciación-Llunell, Ariadna (Hospital Universitari Vall d'Hebron)
Sanz-Martínez, María Teresa (Hospital Universitari Vall d'Hebron)
Viñas-Giménez, Laura (Hospital Universitari Vall d'Hebron)
Selva O'Callaghan, Albert (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia

Data:	2021
Resum:	(1) Background: Myositis specific antibodies (MSA) represent important diagnostic and stratification tools in idiopathic inflammatory myositis (IIM) patients. Here we aimed to evaluate the clinical performance of MSA profiled by a novel particle based multi-analyte technology (PMAT) in IIM and subsets thereof. (2) Methods: 264 IIM patients and 200 controls were tested for MSA using PMAT (Inova Diagnostics, research use only). Diagnostic performance was analyzed and composite scores were generated. (3) Results: The sensitivity/specificity of the individual MSA were: 19. 7%/100% (Jo-1), 7. 2%/100. 0% (Mi-2), 3. 0%/99. 0% (NXP2), 3. 8%/100. 0% (SAE), 2. 7%/100. 0% (PL-7), 1. 9%/99. 5 (PL-12), 1. 1%/100. 0% (EJ), 15. 5%/99. 5% (TIF1γ), 8. 3%/98. 5% (MDA5), 6. 1%/99. 0% (HMGCR) and 1. 9%/98. 5% (SRP). Of all IIM patients, 180/264 tested positive for at least one of the MSAs. In the individual control group, 12/200 (6. 0%) tested positive for at least one MSA, most of which had levels close to the cut-off (except one SRP and one PL-12). Only 6/264 (2. 3%) IIM patients were positive for more than one antibody (MDA5/HMGCR, EJ/PL-7, 2 x MDA5/TIF1γ, EJ/SAE, SAE/TIF1γ). The overall sensitivity was 68. 2% paired with a specificity of 94. 0%, leading to an odds ratio of 33. 8. The composite scores showed good discrimination between subgroups (e. g. , anti-synthetase syndrome). (4) Conclusion: MSA, especially when combined in composite scores (here measured by PMAT), provide value in stratification of patients with IIM.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Myositis ; Autoantibodies ; Diagnosis ; Polymyositis ; Dermatomyositis ; Immunoassay
Publicat a:	Diagnostics, Vol. 11 (november 2021) , ISSN 2075-4418

DOI: 10.3390/diagnostics11122246
PMID: 34943483

14 p, 2.8 MB

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