Combined genomics to discover genes associated with tolerance to soil carbonate
Busoms, Silvia (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Pérez-Martín, Laura (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Terés, Joana (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Huang, Xin-Yuan (Nanjing Agricultural University. State Key Laboratory of Crop Genetics and Germplasm Enhancement)
Yant, Levi (University of Nottingham)
Tolrà Pérez, Roser (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Salt, David E. (University of Nottingham)
Poschenrieder, Charlotte (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)

Data:	2023
Resum:	Carbonate-rich soils limit plant performance and crop production. Previously, local adaptation to carbonated soils was detected in wild Arabidopsis thaliana accessions, allowing the selection of two demes with contrasting phenotypes: A1 (carbonate tolerant, c+) and T6 (carbonate sensitive, c−). Here, A1 and T6 seedlings were grown hydroponically under control (pH 5. 9) and bicarbonate conditions (10 mM NaHCO, pH 8. 3) to obtain ionomic profiles and conduct transcriptomic analysis. In parallel, A1 and T6 parental lines and their progeny were cultivated on carbonated soil to evaluate fitness and segregation patterns. To understand the genetic architecture beyond the contrasted phenotypes, a bulk segregant analysis sequencing (BSA-Seq) was performed. Transcriptomics revealed 208 root and 2503 leaf differentially expressed genes in A1 versus T6 comparison under bicarbonate stress, mainly involved in iron, nitrogen and carbon metabolism, hormones and glycosylates biosynthesis. Based on A1 and T6 genome contrasts and BSA-Seq analysis, 69 genes were associated with carbonate tolerance. Comparative analysis of genomics and transcriptomics discovered a final set of 18 genes involved in bicarbonate stress responses that may have relevant roles in soil carbonate tolerance.
Ajuts:	Agencia Estatal de Investigación PID2019-104000RB-I00
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Plant, cell & environment, (August 2023) , p. 1-13, ISSN 1365-3040

DOI: 10.1111/pce.14691

13 p, 3.3 MB

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