000283592 001 __ 283592
000283592 005 __ 20231025110015.0
000283592 024 7_ $2 doi $a 10.1016/j.scitotenv.2023.166556
000283592 024 8_ $9 scholar $9 driver $9 artpubuab $a oai:ddd.uab.cat:283592
000283592 035 __ $a oai:egreta.uab.cat:publications/925507a5-732e-41a9-882f-b8774ff32544
000283592 035 __ $9 pure_id $a 371078496
000283592 035 __ $9 pmid $a 37633389
000283592 035 __ $9 scopus_id $a 85169604264
000283592 035 __ $9 articleid $a 18791026v904p166556
000283592 041 __ $a eng
000283592 100 1_ $0 0000-0003-3278-0542 $a Güneş, Merve $u Akdeniz University. Department of Biology
000283592 245 10 $a Morphologically different hydroxyapatite nanoparticles exert differential genotoxic effects in Drosophila
000283592 251 __ $1 http://purl.org/coar/version/c_970fb48d4fbd8a85 $2 openaire4 $9 VoR $a Versió publicada
000283592 260 __ $c 2023
000283592 500 __ $a Altres ajuts: acords transformatius de la UAB
000283592 520 __ $a Hydroxyapatite (HAP) occurs naturally in sedimentary and metamorphic rocks and constitutes the hard structures in many organisms. Since synthetic nano-sized HAP (HAP-NPs) are used in orthopedic applications and for heavy metal remediation in aquatic and terrestrial media, both environment and humans are exposed to them. Due to the concerns about their potential hazards, the genotoxic effects that round/rod forms of HAP-NPs were investigated in Drosophila using the wing-spot and the comet assays. Furthermore, caspase activities were evaluated to examine the activation of cell death pathways. As a novelty, the expression of 36 genes involved in DNA repair was investigated, as a tool to indirectly determine DNA damage induction. Obtained sizes were 35-60 nm (roundHAP-NPs) and 45-90 nm (rodHAP-NPs) with a low Zeta-potential (-1.65 and 0.37 mV, respectively). Genotoxicity was detected in the wing-spot (round form), and in the comet assay (round and rod-like HA-NPs). In addition, increased expression of Caspases 3/7, 8, and 9 activities were observed. For both HAP forms, increased changes in the expression were observed for mismatch repair genes, while decreased expression was observed for genes involved in ATM, ATR, and cell cycle pathways. The observed changes in the repair pathways would reinforce the view that HAP-NPs have genotoxic potential, although more markedly in the round form. Thus, the environmental presence of engineered nanoparticles, including HAPs, raises concerns about potential effects on human health. It is essential that the effects of their use are carefully assessed and monitored to ensure safety and to mitigate any potential adverse effects.
000283592 540 __ $9 open access $a Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. $u https://creativecommons.org/licenses/by/4.0/
000283592 546 __ $a Anglès
000283592 599 __ $a recerca
000283592 653 __ $a Caspase
000283592 653 __ $a DNA repair
000283592 653 __ $a Drosophila melanogaster
000283592 653 __ $a Gene expression
000283592 653 __ $a Single-cell gel electrophoresis
000283592 653 __ $a Somatic mutation and recombination
000283592 655 _7 $1 http://purl.org/coar/resource_type/c_6501 $2 openaire4 $a Article $c literature
000283592 700 1_ $0 0000-0002-9694-5839 $a Yalçın, Burçin $u Akdeniz University. Department of Biology
000283592 700 1_ $0 0000-0003-1657-6808 $a Burgazlı, Ayşen Yağmur $u Akdeniz University. Department of Biology
000283592 700 1_ $0 0000-0003-4597-8320 $a Tagorti, Ghada $u Akdeniz University. Department of Biology
000283592 700 1_ $0 0000-0001-9855-7437 $a Yavuz, Emre $u Akdeniz University. Department of Chemistry
000283592 700 1_ $0 0000-0002-1965-5774 $a Akarsu, Esin $u Akdeniz University. Department of Chemistry
000283592 700 1_ $0 0000-0002-3227-6680 $a Kaya, Nuray $u Akdeniz University. Department of Biology
000283592 700 1_ $0 0000-0001-7891-357X $a Marcos Dauder, Ricardo $u Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia
000283592 700 1_ $0 0000-0002-0491-9781 $a Kaya, Bülent $u Akdeniz University. Department of Biology
000283592 773 __ $g Vol. 904 (December 2023), art. 166556 $t Science of the total environment $x 1879-1026
000283592 856 40 $p 15 $s 9663650 $u https://ddd.uab.cat/pub/artpub/2023/b8774ff32544/1-s2.0-S0048969723051811-main.pdf
000283592 973 __ $f 166556 $m 12 $v 904 $x 18791026_a2023v904 $y 2023
000283592 980 __ $a ARTPUB $b UAB