Web of Science: 3 cites, Scopus: 3 cites, Google Scholar: cites
Morphologically different hydroxyapatite nanoparticles exert differential genotoxic effects in Drosophila
Güneş, Merve (Akdeniz University. Department of Biology)
Yalçın, Burçin (Akdeniz University. Department of Biology)
Burgazlı, Ayşen Yağmur (Akdeniz University. Department of Biology)
Tagorti, Ghada (Akdeniz University. Department of Biology)
Yavuz, Emre (Akdeniz University. Department of Chemistry)
Akarsu, Esin (Akdeniz University. Department of Chemistry)
Kaya, Nuray (Akdeniz University. Department of Biology)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Kaya, Bülent (Akdeniz University. Department of Biology)

Data: 2023
Resum: Hydroxyapatite (HAP) occurs naturally in sedimentary and metamorphic rocks and constitutes the hard structures in many organisms. Since synthetic nano-sized HAP (HAP-NPs) are used in orthopedic applications and for heavy metal remediation in aquatic and terrestrial media, both environment and humans are exposed to them. Due to the concerns about their potential hazards, the genotoxic effects that round/rod forms of HAP-NPs were investigated in Drosophila using the wing-spot and the comet assays. Furthermore, caspase activities were evaluated to examine the activation of cell death pathways. As a novelty, the expression of 36 genes involved in DNA repair was investigated, as a tool to indirectly determine DNA damage induction. Obtained sizes were 35-60 nm (roundHAP-NPs) and 45-90 nm (rodHAP-NPs) with a low Zeta-potential (-1. 65 and 0. 37 mV, respectively). Genotoxicity was detected in the wing-spot (round form), and in the comet assay (round and rod-like HA-NPs). In addition, increased expression of Caspases 3/7, 8, and 9 activities were observed. For both HAP forms, increased changes in the expression were observed for mismatch repair genes, while decreased expression was observed for genes involved in ATM, ATR, and cell cycle pathways. The observed changes in the repair pathways would reinforce the view that HAP-NPs have genotoxic potential, although more markedly in the round form. Thus, the environmental presence of engineered nanoparticles, including HAPs, raises concerns about potential effects on human health. It is essential that the effects of their use are carefully assessed and monitored to ensure safety and to mitigate any potential adverse effects.
Nota: Altres ajuts: acords transformatius de la UAB
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Caspase ; DNA repair ; Drosophila melanogaster ; Gene expression ; Single-cell gel electrophoresis ; Somatic mutation and recombination
Publicat a: Science of the total environment, Vol. 904 (December 2023) , art. 166556, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2023.166556
PMID: 37633389


15 p, 9.2 MB

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 Registre creat el 2023-10-14, darrera modificació el 2023-10-25



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