Toxicological Profiling and Long-Term Effects of Bare, PEGylated- and Galacto-Oligosaccharide-Functionalized Mesoporous Silica Nanoparticles
Barguilla, Irene (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Candela-Noguera, Vicente (Universitat Politècnica de València)
Oliver, Patrick (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Annangi, Balasubramanyam (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Díez, Paula (Universitat Politècnica de València)
Aznar, Elena (Universitat Politècnica de València)
Martínez-Máñez, Ramón (Universitat Politècnica de València)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marcos Martínez, Maria Dolores (Universitat Politècnica de València)

Date:	2023
Abstract:	Mesoporous silica nanoparticles (MSNs) are amongst the most used nanoparticles in biomedicine. However, the potentially toxic effects of MSNs have not yet been fully evaluated, being a controversial matter in research. In this study, bare MSNs, PEGylated MSNs (MSNs-PEG), and galacto-oligosaccharide-functionalized MSNs (MSNs-GAL) are synthesized and characterized to assess their genotoxicity and transforming ability on human lung epithelial BEAS-2B cells in short- (48 h) and long-term (8 weeks) exposure scenarios. Initial short-term treatments show a dose-dependent increase in genotoxicity for MSNs-PEG-treated cells but not oxidative DNA damage for MSNs, MSNs-PEG, or for MSNs-GAL. In addition, after 8 weeks of continuous exposure, neither induced genotoxic nor oxidative DNA is observed. Nevertheless, long-term treatment with MSNs-PEG and MSNs-GAL, but not bare MSNs, induces cell transformation features, as evidenced by the cell's enhanced ability to grow independently of anchorage, to migrate, and to invade. Further, the secretome from cells treated with MSNs and MSNs-GAL, but not MSNs-PEG, shows certain tumor-promoting abilities, increasing the number and size of HeLa cell colonies formed in the indirect soft-agar assay. These results show that MSNs, specifically the functionalized ones, provoke some measurable adverse effects linked to tumorigenesis. These effects are in the order of other nanomaterials, such as carbon nanotubes or cerium dioxide nanoparticles, but they are lower than those provoked by some approved drugs, such as doxorubicin or dexamethasone.
Grants:	Agencia Estatal de Investigación PID2021-126304OB-C41 Agencia Estatal de Investigación PID2021-128141OB-C22 Agencia Estatal de Investigación PID2020-116789RB-C43 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731 Ministerio de Ciencia e Innovación CB06/01/2012 European Commission 965196 Ministerio de Educación, Cultura y Deporte FPU15/02753
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Mesoporous silica nanoparticles ; Long-term effects ; Toxicological profiling
Published in:	International journal of molecular sciences, Vol. 24, Issue 22 (November 2023) , art. 16158, ISSN 1422-0067

DOI: 10.3390/ijms242216158
PMID: 38003350

14 p, 1.6 MB

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