Graphene Oxide Nanosheets Hamper Glutamate Mediated Excitotoxicity and Protect Neuronal Survival In An In vitro Stroke Model
Tortella, Lorenza (International School for Advanced Studies)
Santini, Irene (International School for Advanced Studies)
Lozano, Neus (Institut Català de Nanociència i Nanotecnologia)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Cellot, Giada (International School for Advanced Studies)
Ballerini, Laura (International School for Advanced Studies)

Fecha:	2023
Resumen:	Small graphene oxide (s-GO) nanosheets reversibly downregulate central nervous system (CNS) excitatory synapses, with potential developments as future therapeutic tools to treat neuro-disorders characterized by altered glutamatergic transmission. Excitotoxicity, namely cell death triggered by exceeding ambient glutamate fueling over-activation of excitatory synapses, is a pathogenic mechanism shared by several neural diseases, from ischemic stroke to neurodegenerative disorders. In this work, CNS cultures were exposed to oxygen-glucose deprivation (OGD) to mimic ischemic stroke in vitro, and it is show that the delivery of s-GO following OGD, during the endogenous build-up of secondary damage and excitotoxicity, improved neuronal survival. In a different paradigm, excitotoxicity cell damage was reproduced through exogenous glutamate application, and s-GO co-treatment protected neuronal integrity, potentially by directly downregulating the synaptic over-activation brought about by exogenous glutamate. This proof-of-concept study suggests that s-GO may find novel applications in therapeutic developments for treating excitotoxicity-driven neural cell death.
Ayudas:	Agencia Estatal de Investigación CEX2021-001214-S Ministerio de Ciencia e Innovación SEV-2017-0706 European Commission 881603
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Chemistry (Weinheim), Vol. 29, Issue 67 (December 2023) , art. e202301762, ISSN 1521-3765

DOI: 10.1002/chem.202301762

10 p, 1.5 MB

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