Association of serum and fecal microRNA profiles in cats with gastrointestinal cancer and chronic inflammatory enteropathy
Brogaard, Louise (Technical University of Denmark)
Lyngby, Janne G. (University of Copenhagen)
Kristensen, Annemarie T. (University of Copenhagen)
Fredholm, Merete (University of Copenhagen)
Bjørnvad, Charlotte R. (University of Copenhagen)
Salavati Schmitz, Silke (University of Edinburgh. Royal School of Veterinary Studies)
Skancke, Ellen (Norwegian University of the Life Sciences)
Morris, Joanna S. (University of Glasgow. School of Veterinary Medicine)
Dupont, Nana (University of Copenhagen)
Argyle, David (University of Edinburgh. Royal School of Veterinary Studies)
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Spohr, Anette (Evidensia Faxe Animal Hospital)
Graarup-Hansen, Kasper (Evidensia Karlslunde Animal Hospital)
Nielsen, Lise N. (University of Copenhagen)
Cirera, Susanna (University of Copenhagen)

Fecha:	2023
Resumen:	Differentiation of gastrointestinal cancer (GIC) from chronic inflammatory enteropathies (CIE) in cats can be challenging and often requires extensive diagnostic testing. MicroRNAs (miRNAs) have promise as non-invasive biomarkers in serum and feces for diagnosis of GIC. Cats with GIC will have serum and fecal miRNA profiles that differ significantly from healthy cats and cats with CIE. Identify serum and fecal miRNAs with diagnostic potential for differentiation between cats with GIC and CIE as compared to healthy cats. Ten healthy cats, 9 cats with CIE, and 10 cats with GIC; all client-owned. Cats were recruited for an international multicenter observational prospective case-control study. Serum and feces were screened using small RNA sequencing for miRNAs that differed in abundance between cats with GIC and CIE, and healthy cats. Diagnostic biomarker potential of relevant miRNAs from small RNA sequencing and the literature was confirmed using reverse transcription quantitative real-time PCR (RT-qPCR). Serum miR-223-3p was found to distinguish between cats with GIC and CIE with an area under the curve (AUC) of 0. 9 (95% confidence interval [CI], 0. 760-1. 0), sensitivity of 90% (95% CI, 59. 6-99. 5%), and specificity of 77. 8% (95% CI, 45. 3-96. 1%). Serum miR-223-3p likewise showed promise in differentiating a subgroup of cats with small cell lymphoma (SCL) from those with CIE. No fecal miRNAs could distinguish between cats with GIC and CIE. Serum miR-223-3p potentially may serve as a noninvasive diagnostic biomarker of GIC in cats, in addition to providing a much needed tool for the differentiation of CIE and SCL.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Biomarker ; CIE ; Lymphoma ; Mirna ; Qpcr ; Small RNA sequencing
Publicado en:	Journal of Veterinary Internal Medicine, Vol. 37 (july 2023) , p. 1738-1749, ISSN 1939-1676

DOI: 10.1111/jvim.16813
PMID: 37486176

12 p, 1.4 MB

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