Biochemical analysis and optimization of inhibition and adsorption phenomena in glass-silicon PCR-chips
Erill, Ivan (Centro Nacional de Microelectrónica (Espanya))
Campoy Sánchez, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Erill, Nadina (Hospital de Barcelona. Laboratori de Biopatologia Molecular)
Barbé García, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Aguiló Llobet, Jordi (Centro Nacional de Microelectrónica (Espanya))

Date:	2003
Abstract:	The use of glass-silicon chips for PCR analysis has been widely reported in the last decade, but there have been few systematic efforts to pin down the biochemical problems such systems bring forth. Here we report a systematic analysis of material-related inhibition and adsorption phenomena in glass-silicon PCR-chips. The results suggest that the previously reported inhibition of PCR by silicon-related materials stems mainly from the adsorption of Taq polymerase at chip walls due to increased surface-to-volume ratios, and not from a straight chemical action of silicon-related materials on the PCR-mix. In contrast to Taq polymerase, DNA is not adsorbed in noticeable amounts. The net effect of polymerase adsorption can be counteracted by the addition of a titrated amount of competing protein bovine serum albumin (BSA) and the ensuing reactions can be kinetically optimized in chips to yield effective amplifications in the whereabouts of 20 min.
Grants:	Ministerio de Ciencia y Tecnología BMC2001-2065 Agència de Gestió d'Ajuts Universitaris i de Recerca 2001/SGR-206
Note:	Altres ajuts: this work was partly funded by the Consejo de Investigaciones Cientı́ficas (CSIC), by Grant TIC97-0569 from the Comisión Interministerial de Ciencia y Tecnologı́a (CICYT)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Published in:	Sensors and actuators. B, Chemical, Vol. 96, issue 3 (Des. 2003) , p. 685-692, ISSN 1873-3077

DOI: 10.1016/S0925-4005(03)00522-7

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