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Pàgina inicial > Articles > Articles publicats > Identification of meiotic anomalies with multiplex fluorescence in situ hybridization : |
Data: | 2004 |
Descripció: | 6 pàg. |
Resum: | To characterize meiotic anomalies in infertile men by multiplex fluorescence in situ hybridization (M-FISH) and to determine whether synaptic problems affect specific bivalents or whether anomalies are random. Analysis of meiotic preparations with standard techniques and M-FISH. Assisted reproduction centers and Universitat Autònoma de Barcelona. Three fertile men undergoing vasectomy, four sterile patients with oligoasthenoteratozoospermia, and one patient with a Robertsonian translocation t(13;14). Unilateral testicular biopsy in controls and patients with oligoasthenoteratozoospermia and collection of a semen sample from the translocation carrier. Identification of bivalents in metaphase I and chromosomes in metaphase II and characterization of chromosome abnormalities. All bivalents in metaphase I and all chromosomes in metaphase II could be identified. In controls and in one patient with oligoasthenoteratozoospermia, meiosis was normal. Other patients with oligoasthenoteratozoospermia showed different types of anomaly: desynapsis, breaks, precocious XY separation, or cryptic reorganizations. The Robertsonian translocation t(13;14) was easily identified. Results confirm the high incidence of synaptic errors in oligoasthenoteratozoospermia patients. Bivalents in metaphase I and chromosomes in metaphase II were individually identifiable. Nondisjunctional errors or small reorganizations overlooked in classic meiotic preparations were identified. Synaptic anomalies seem to affect meiotic bivalents at random. © 2004 by American Society for Reproductive Medicine. |
Ajuts: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2001/SGR-00202 Ministerio de Ciencia y Tecnología SAF2003-04312 |
Nota: | Altres ajuts: supported by Project 161275 (Universitat Autònoma de Barcelona, Barcelona, Spain) |
Drets: | Tots els drets reservats. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Matèria: | Meiosis ; Chromosome reorganizations ; Multiplex FISH ; Synaptic anomalies |
Publicat a: | Fertility and Sterility, Vol. 82, no. 3 (September 2004) , p. 712-717, ISSN 1556-5653 |
Postprint 23 p, 766.4 KB |