Acrocentric bivalents positioned preferentially nearby to the XY pair in metaphase i human spermatocytes
Sarrate Navas, Zaida (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Blanco, Joan (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Vidal, Francesca (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date:	2012
Abstract:	Objective: To analyze whether the preferential proximity between acrocentric bivalents and the XY pair described at pachytene was maintained in metaphase I human spermatocytes. Design: Proximity frequencies of autosomic bivalents to the sex bivalent were evaluated with the analysis of meiotic preparations combining sequentially standard techniques and multiplex fluorescence in situ hybridization. Setting: Assisted reproduction centers. Patient(s): Thirty-seven men consulting for fertility problems. Intervention(s): Unilateral testicular biopsies. Main Outcome Measure(s): Proximity frequencies analysis to the XY pair, evaluated individually and grouping bivalents, was carried out using a logistical regression model with repeated measures. Result(s): Bivalents 22 and 15 were observed more frequently near to the sex bivalent than the others. Significant interindividual differences were not observed. Conclusion(s): Results suggest that bivalents distribution to the metaphase plate is nonrandom. The maintenance of the acrocentric chromosomes' proximity to the sex bivalent from pachytene to metaphase I would indicate that the relative bivalents position would be notably preserved. The observation of non-interindividual variability, despite different infertility etiology, suggests that the nuclear organization pattern remains largely unaffected even if spermatogenesis is compromised.
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Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Acrocentric chromosomes ; Chromosome territories ; Human spermatocytes ; Metaphase I ; XY bivalent ; SDG 3 - Good Health and Well-being
Published in:	Fertility and Sterility, Vol. 98 Núm. 5 (2012) , p. 1241-1245, ISSN 1556-5653

DOI: 10.1016/j.fertnstert.2012.07.1110

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