Modulation of autoimmune arthritis severity in mice by apolipoprotein E (ApoE) and cholesterol
Alvarez, Pilar (Instituto de Biomedicina y Biotecnología de Cantabria)
Genre, Fernanda (Universidad de Cantabria)
Iglesias, Marcos (Universidad de Cantabria)
Augustín Rodríguez, Juan Jesús (Universidad de Cantabria)
Tamayo, Esther (Universidad de Cantabria)
Escolà-Gil, Joan Carles (Institut d'Investigació Biomèdica Sant Pau)
Lavín Gómez, Bernardo (Hospital Universitario Marqués de Valdecilla (Santander, Cantabria))
Blanco Vaca, Francisco (Institut d'Investigació Biomèdica Sant Pau)
Merino Pérez, Ramón (Universidad de Cantabria)
Merino Pérez, Jesús (Universidad de Cantabria)
Universitat Autònoma de Barcelona

Fecha:	2016
Resumen:	Apolipoprotein E (ApoE) deficiency promoted an exacerbation of autoimmune arthritis in mice by inducing proinflammatory immune responses. In this study we analysed the contribution of hypercholesterolaemia and/or the absence of ApoE anti-inflammatory properties, unrelated to its function in the control of cholesterol metabolism, towards the acceleration of arthritis in these mutant animals. The induction and severity of collagen type II-induced arthritis (CIA) were compared for B10. RIII wild-type (WT), B10. RIII. ApoE, B10. RIII. ApoE and B10. RIII. low-density lipoprotein receptor (LDLR) mice with different concentrations of circulating ApoE and cholesterol. A 50-70% reduction in serum levels of ApoE was observed in heterozygous B10. RIII. ApoE mice in comparison to B10. RIII. WT, although both strains of mice exhibited similar circulating lipid profiles. This ApoE reduction was associated with an increased CIA severity that remained lower than in homozygous B10. RIII. ApoE mice. An important rise in circulating ApoE concentration was observed in hypercholesterolaemic B10. RIII. LDLR mice fed with a normal chow diet, and both parameters increased further with an atherogenic hypercholesterolaemic diet. However, the severity of CIA in B10. RIII. LDLR mice was similar to that of B10. RIII. WT controls. In conclusion, by comparing the evolution of CIA between several strains of mutant mice with different levels of serum ApoE and cholesterol, our results demonstrate that both hypercholesterolaemia and ApoE regulate the intensity of in-vivo systemic autoimmune responses.
Ayudas:	Ministerio de Economía y Competitividad SAF2012-34059 Ministerio de Economía y Competitividad SAF2014-55088-R Ministerio de Ciencia e Innovación IPT2011-1527-010000
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	ApoE ; LDLR ; Collagen type II-induced arthritis ; Hypercholesterolaemia
Publicado en:	Clinical and Experimental Immunology, Vol. 186 Núm. 3 (january 2016) , p. 292-303, ISSN 1365-2249

DOI: 10.1111/cei.12857
PMID: 27571306

12 p, 742.1 KB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut de Recerca Sant Pau
Artículos > Artículos de investigación
Artículos > Artículos publicados