Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
Rossetti, Marianna (Institut Català de Nanociència i Nanotecnologia)
Srisomwat, Chawin (Chulalongkorn University. Department of Chemistry)
Urban, Massimo (Universitat Autònoma de Barcelona)
Rosati, Giulio (Institut Català de Nanociència i Nanotecnologia)
Maroli, Gabriel (Institut Català de Nanociència i Nanotecnologia)
Yaman Akbay, Hatice Gödze (Institut Català de Nanociència i Nanotecnologia)
Chailapakul, Orawon (Chulalongkorn University. Department of Chemistry)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2024
Abstract:	Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.
Grants:	Ministerio de Ciencia e Innovación SEV-2017-0706 Agencia Estatal de Investigación PID2021-124795NB-I00
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Electrochemical RNA detection ; Inkjet printed electrodes ; Battery-free NFC potentiostat ; Multiplexed biosensors ; Wireless ; Nanoporous gold
Published in:	Biosensors & bioelectronics, Vol. 250 (April 2024) , art. 116079, ISSN 1873-4235

DOI: 10.1016/j.bios.2024.116079

9 p, 4.2 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles