Digital dermatitis-associated Treponema species detection and quantification in migratory tundra caribou (Rangifer tarandus)
Petersen Dias, Angelica (University of Calgary)
Fernández Aguilar, Xavier (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals.)
De Buck, Jeroen (University of Calgary)
Kutz, Susan (University of Calgary)
Arrazuria, Rakel (University of Calgary)

Date:	2024
Abstract:	Treponema spp. are associated with infectious lameness in livestock and wild ruminants. While extensive research has been conducted on cattle, investigations in wild ruminants are scarce. Hoof disease is common in caribou populations (Rangifer tarandus), but investigations are limited due to the remoteness of the Arctic. Our study aimed to assess the presence of Treponema spp. associated with bovine digital dermatitis in caribou. DNA was extracted from coronary band tissues from forty-eight caribou without visible hoof lesions and analyzed using two PCR methods (qPCR and nPCR). Treponema spp. were detected in low copy numbers/mg of tissue (3. 6 to 6. 6 × 10). T. phagedenis was the most prevalent and abundant species in 58% of samples by qPCR, followed by T. medium (44%), and T. pedis (10%). The qPCR and nPCR agreement ranged between 65% and 75% (Cohen's kappa 0. 22-0. 51). Sanger sequencing of thirteen nPCR products confirmed that treponemes in caribou are remarkably similar to those found in domestic ruminants and wild elk. Our study highlights the colonization of treponemes in healthy hooves of a wild ruminant in the Arctic, where there is no presence of livestock, and expands knowledge on the host range and distribution of treponemes. These findings also emphasize the need for further research into the multifactorial nature of treponema-associated hoof diseases and the putative role of treponemes in infectious lameness affecting caribou.
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Arctic health ; Digital dermatitis ; Infectious lameness ; Qpcr ; Treponema-associated hoof disease ; Wildlife
Published in:	Research in veterinary science, Vol. 171 (may 2024) , p. 105210, ISSN 1532-2661

DOI: 10.1016/j.rvsc.2024.105210

4 p, 596.8 KB

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