Peptide-based biosensing approaches for targeting breast cancer-derived exosomes
da Fonseca Alves, Rafael (Universitat Autònoma de Barcelona. Departament de Química)
Pallarès-Rusiñol, Arnau (Universitat Autònoma de Barcelona. Departament de Química)
Rossi, Rosanna (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Martí, Mercè (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Rezende Vaz, Emilia (Universidade Estadual Paulista)
Gonçalves de Araújo, Thaise (Universidade Federal de Uberlândia)
Sotomayor, Maria (Universidade Federal de Uberlândia)
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química)

Fecha:	2024
Resumen:	Exosomes are nanovesicles present in all the biological fluids, making them attractive as non-invasive biomarkers for diseases like cancer, among many others. However, exosomes are complex to separate and detect, requiring comprehensive molecular characterization for their routine use in diagnostics. This study explores the use of peptides as cost-effective and stable alternatives to antibodies for exosome binding. To achieve that, phage display technology was employed to select peptides with high specificity for target molecules in exosomes. Specifically, a selected peptide was evaluated for its ability to selectively bind breast cancer-derived exosomes. Proteomic analysis identified 38 protein candidates targeted by the peptide on exosome membranes. The binding of the peptide to breast cancer-derived exosomes was successfully demonstrated by flow cytometry and magneto-actuated immunoassays. Furthermore, an electrochemical biosensor was also tested for breast cancer-derived exosome detection and quantification. The peptide demonstrated effective binding to exosomes from aggressive cancer cell lines, offering promising results in terms of specificity and recovery. This research shows potential for developing rapid, accessible diagnostic tools for breast cancer, especially in low-resource healthcare settings.
Ayudas:	Agencia Estatal de Investigación PID2019-106625RB-I00 Agencia Estatal de Investigación PID2022-136453OB-I00 Agencia Estatal de Investigación PDC2022-133363 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00124
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Exosomes ; Magnetic separation ; Mitovesicles ; Peptide-based biosensors ; Phage display ; Proteomic analysis
Publicado en:	Biosensors & bioelectronics, Vol. 255 (July 2024) , art. 116211, ISSN 1873-4235

DOI: 10.1016/j.bios.2024.116211
PMID: 38537428

8 p, 4.5 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
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