Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
Bovio-Winkler, P. (Instituto de Investigaciones Biológicas Clemente Estable)
Guerrero, L.D. (Instituto de Investigaciones en Ingeniería Genética Y Biología Molecular "Dr Héctor N. Torres" (INGEBI-CONICET))
Erijman, L. (Instituto de Investigaciones en Ingeniería Genética Y Biología Molecular "Dr Héctor N. Torres" (INGEBI-CONICET))
Oyarzúa, P. (Universitat Autònoma de Barcelona)
Suárez Ojeda, María Eugenia (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Cabezas, A. (Instituto Tecnológico Regional Centro Sur)
Etchebehere, C. (Instituto de Investigaciones Biológicas Clemente Estable)

Data:	2023
Resum:	The phylum Chloroflexi is highly abundant in a wide variety of wastewater treatment bioreactors. It has been suggested that they play relevant roles in these ecosystems, particularly in degrading carbon compounds and on structuring flocs or granules. Nevertheless, their function is not yet well understood as most species have not been isolated in axenic cultures. Here we used a metagenomic approach to investigate Chloroflexi diversity and their metabolic potential in three environmentally different bioreactors: a methanogenic full-scale reactor, a full-scale activated sludge reactor and a lab scale anammox reactor. Differential coverage binning approach was used to assemble the genomes of 17 new Chloroflexi species, two of which are proposed as new Candidatus genus. In addition, we recovered the first representative genome belonging to the genus 'Ca. Villigracilis'. Even though samples analyzed were collected from bioreactors operating under different environmental conditions, the assembled genomes share several metabolic features: anaerobic metabolism, fermentative pathways and several genes coding for hydrolytic enzymes. Interestingly, genome analysis from the anammox reactor indicated a putative role of Chloroflexi in nitrogen conversion. Genes related to adhesiveness and exopolysaccharides production were also detected. Complementing sequencing analysis, filamentous morphology was detected by Fluorescent in situ hybridization. Our results suggest that Chloroflexi participate in organic matter degradation, nitrogen removal and biofilm aggregation, playing different roles according to the environmental conditions.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Activated sludge ; Anammox ; Chloroflexi ; Metagenome assembled genomes ; Methanogenic reactors
Publicat a:	BMC Microbiology, Vol. 23 Núm. 1 (december 2023) , p. 45, ISSN 1471-2180

DOI: 10.1186/s12866-023-02765-5
PMID: 36809975

19 p, 5.4 MB

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