Redefining the Role of ADAM17 in Renal Proximal Tubular Cells and Its Implications in an Obese Mouse Model of Pre-Diabetes
Palau, Vanesa (Institut Hospital del Mar d'Investigacions Mèdiques)
Villanueva, Sofia (Institut Hospital del Mar d'Investigacions Mèdiques)
Jarrín, Josué (Institut Hospital del Mar d'Investigacions Mèdiques)
Benito, David (Institut Hospital del Mar d'Investigacions Mèdiques)
Márquez, Eva (Institut Hospital del Mar d'Investigacions Mèdiques)
Rodríguez García, Eva (Institut Hospital del Mar d'Investigacions Mèdiques)
Soler, María José (Hospital Universitari Vall d'Hebron)
Oliveras i Serrano, Anna (Institut Hospital del Mar d'Investigacions Mèdiques)
Gimeno Beltran, Javier (Hospital del Mar (Barcelona, Catalunya))
Sans, Laia (Institut Hospital del Mar d'Investigacions Mèdiques)
Soler, María José (Institut Hospital del Mar d'Investigacions Mèdiques)
Pascual, Julio (Institut Hospital del Mar d'Investigacions Mèdiques)
Barrios, Clara (Institut Hospital del Mar d'Investigacions Mèdiques)
Riera Oliva, Marta (Institut Hospital del Mar d'Investigacions Mèdiques)
Universitat Autònoma de Barcelona

Fecha:	2021
Resumen:	Acute and chronic kidney lesions induce an increase in A Disintegrin And Metalloproteinase domain 17 (ADAM17) that cleaves several transmembrane proteins related to inflammatory and fibrotic pathways. Our group has demonstrated that renal ADAM17 is upregulated in diabetic mice and its inhibition decreases renal inflammation and fibrosis. The purpose of the present study was to analyze how Adam17 deletion in proximal tubules affects different renal structures in an obese mice model. Tubular Adam17 knockout male mice and their controls were fed a high-fat diet (HFD) for 22 weeks. Glucose tolerance, urinary albumin-to-creatinine ratio, renal histology, and pro-inflammatory and pro-fibrotic markers were evaluated. Results showed that wild-type mice fed an HFD became obese with glucose intolerance and renal histological alterations mimicking a pre-diabetic condition; consequently, greater glomerular size and mesangial expansion were observed. Adam17 tubular deletion improved glucose tolerance and protected animals against glomerular injury and prevented podocyte loss in HFD mice. In addition, HFD mice showed more glomerular macrophages and collagen accumulation, which was prevented by Adam17 deletion. Galectin-3 expression increased in the proximal tubules and glomeruli of HFD mice and ameliorated with Adam17 deletion. In conclusion, Adam17 in proximal tubules influences glucose tolerance and participates in the kidney injury in an obese pre-diabetic murine model. The role of ADAM17 in the tubule impacts on glomerular inflammation and fibrosis.
Ayudas:	Ministerio de Economía y Competitividad PI16/00620 Ministerio de Economía y Competitividad RD16/0009/0013 Instituto de Salud Carlos III PI17/00257
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	ADAM17 ; Obesity ; Pre-diabetes ; Renal proximal tubular cells
Publicado en:	International journal of molecular sciences, Vol. 22 (december 2021) , ISSN 1422-0067

DOI: 10.3390/ijms222313093
PMID: 34884897

15 p, 3.4 MB

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