Measurement of haptoglobin in saliva of cows : Validation of an assay and a pilot study of its potential application
Botía, María (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
López-Arjona, Marina (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Escribano Tortosa, Damián (Universidad de Murcia. Departamento de Producción Animal)
Contreras-Aguilar, M. D (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Vallejo-Mateo, Pedro Javier (Universidad de Murcia. Departamento de Medicina y Cirugía Animal)
Cerón, José Joaquín (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Martínez-Subiela, Silvia (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)

Date:	2023
Abstract:	In recent years, the use of saliva as a matrix for the measurement of biomarkers of health and welfare is gaining importance due to its non-invasive collection. Haptoglobin (Hp) is an acute-phase protein involved in the inflammatory response and changes in its concentration can provide information about the health status of the animals. This study aimed to develop and validate an assay based on luminescent amplification (AlphaLISA technology) for the measurement of Hp in bovine saliva and to study the possible changes in different inflammatory situations such as peripartum period and lameness. The assay proved to be accurate, reliable, and sensitive for the measurement of Hp in cow saliva (coefficient of variation (CV) 7. 57%; coefficient of determination (R) 0. 992; recovery test 105. 15%; lower limit of quantification (LLQ) 7. 9 ng/ml). Significant differences were observed between Hp levels in saliva of cows before (13 days before) and after (7 and 20 days after) calving and at the moment of calving (p < 0. 0001), and between lame and healthy cows (p < 0. 008). In conclusion, this assay can detect Hp in a precise, sensitive, and accurate way in saliva of cows. Future studies with a larger population and different disease conditions should be conducted to determine the potential of Hp as an inflammatory biomarker in cow saliva.
Grants:	European Commission 862919 Ministerio de Ciencia e Innovación FJC2021-047105-I
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Dairy cattle ; Haptoglobin ; Lameness ; Peripartum ; Saliva
Published in:	Research in veterinary science, Vol. 158 (May 2023) , p. 44-49, ISSN 1532-2661

DOI: 10.1016/j.rvsc.2023.03.005

6 p, 1.0 MB

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