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| Home > Articles > Published articles > Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications |
(Institut de Microelectrònica de Barcelona) (Instituto de Salud Carlos III. Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) (Institut Català de Nanociència i Nanotecnologia) (Institut Català de Nanociència i Nanotecnologia) (Instituto de Salud Carlos III. Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina) (Institut Català de Nanociència i Nanotecnologia) (Instituto de Salud Carlos III. Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)| Date: | 2024 |
| Abstract: | Graphene solution-gated field-effect transistors (gSGFETs) offer high potential for chemical and biochemical sensing applications. Among the current trends to improve this technology, the functionalization processes are gaining relevance for its crucial impact on biosensing performance. Previous efforts are focused on simplifying the attachment procedure from standard multi-step to single-step strategies, but they still suffer from overreaction, and impurity issues and are limited to a particular ligand. Herein, a novel strategy for single-step immobilization of chemically modified aptamers with fluorenylmethyl and acridine moieties, based on a straightforward synthetic route to overcome the aforementioned limitations is presented. This approach is benchmarked versus a standard multi-step strategy using thrombin as detection model. In order to assess the reliability of the functionalization strategies 48-gSGFETs arrays are employed to acquire large datasets with multiple replicas. Graphene surface characterization demonstrates robust and higher efficiency in the chemical coupling of the aptamers with the single-step strategy, while the electrical response evaluation validates the sensing capability, allowing to implement different alternatives for data analysis and reduce the sensing variability. In this work, a new tool capable of overcome the functionalization challenges of graphene surfaces is provided, paving the way toward the standardization of gSGFETs for biosensing purposes. |
| Grants: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00495 European Commission 785219 Agencia Estatal de Investigación RYC-2016-21412 Agencia Estatal de Investigación RYC2019-027879-I Agencia Estatal de Investigación RYC2020-030183-I Agencia Estatal de Investigación PID2020-118145RB-I00 Agencia Estatal de Investigación PID2021-126117NA-I00 Agencia Estatal de Investigación MDM-2017-0720 European Commission 881603 Ministerio de Sanidad y Consumo CB06/01/0019 Ministerio de Sanidad y Consumo CB06/01/0049 |
| Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.  |
| Language: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Published in: | Small, Vol. 20, Issue 18 (May 2024) , art. 2308857, ISSN 1613-6829 |
