Distinct responses of active and total bacterial communities to inorganic fertilization in a 30-year experimental site
Ding, Kai (Chinese Academy of Sciences. Institute of Urban Environment)
Su, Xiaoxuan (Chinese Academy of Sciences. Institute of Urban Environment)
Li, Hu (Chinese Academy of Sciences. Institute of Urban Environment)
Dai, Qing-Xia (Hanshan Normal University. Department of Chemistry and Environment Engineering)
Grau Fernández, Oriol (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)

Date:	2022
Abstract:	Soil microbial communities play a vital role in mediating nutrient turnover, thus enhancing growth and development of plants. Understanding the dynamics of microorganisms in soils can provide insight into the influence of fertilization practices on improving soil fertility and plant growth in agricultural ecosystems. In this study, we compared the abundances and compositions of total (DNA-based, 16S rRNA gene) and active (RNA-based, 16S rRNA) bacterial communities at a 30-year experimental site in different inorganic fertilization treatments with different key elements (nitrogen, phosphorus, and potassium). The inorganic fertilizer amendments did not affect the abundance of total bacteria but significantly affected the abundance of active bacteria due to changes in microbial biomass carbon and NH4+-N contents. Cyanobacteria and Proteobacteria, especially for some dominant orders (e. g. Nostocales, Pseudanabaenales and Nitrosomonadales) were the dominant phyla in the active microbial community and differed proportionally in nitrogen and phosphorus fertilized soil. Soil N speciation (e. g. total N, NH4+-N and NO3--N) were the main determinants controlling the Cyanobacteria and Proteobacteria communities. Our results indicated that the unbalanced fertilization could reduce the abundance of active bacteria and significantly changed the dominant phyla compared with balanced fertilization. These findings provided an insight of composition and ratio of nutrient elements including nitrogen, phosphorus and potassium for managing future fertilization regimes in agricultural ecosystem.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Inorganic fertilization ; Total microbes ; Active microbes ; RNA ; Bacterial community
Published in:	Applied Soil Ecology, Vol. 179 (November 2022) , art. 104584, ISSN 1873-0272

DOI: 10.1016/j.apsoil.2022.104584

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CREAF (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Articles > Research articles
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