Multi-compartmentalized electrochemical sensing platforms for monitoring cascade enzymatic reactions
López Fernández, Laia (Universitat Autònoma de Barcelona. Departament de Química)
Bastos Arrieta, Julio (Universitat de Barcelona. Departament d'Enginyeria Química i Química Analítica)
Appelhans, Dietmar (Leibniz-Institut für Polymerforschung (Germany))
Zhou, Yang (Leibniz-Institut für Polymerforschung (Germany))
Moreno Pinilla, Silvia (Leibniz-Institut für Polymerforschung (Germany))
Palet i Ballús, Cristina (Universitat Autònoma de Barcelona. Departament de Química)
Baeza, Mireia (Universitat Autònoma de Barcelona. Departament de Química)

Fecha:	2024
Descripción:	10 pàg.
Resumen:	Designing an electrochemical biosensor with the required features is a complex process involving multiple factors. For instance, nanocomposite materials used ensure the adaptation of the sensor to specific parameters on demand. But beyond making more versatile sensors, these materials likely offer excellent sensing platforms to mimic biological processes (e. g. cell communication) on the electrode surface. For this, developed methods are needed to integrate non-conductive nanoreactors for molecular communication into a biocompatible matrix on electrode surfaces with the request of spatially separated and controlled enzyme localization. Here, we present a novel reduced graphene oxide hybrid material to immobilize polymeric nanoreactors supported by an alginate network as a matrix on the electrode surface. The possibility of introducing cascade reactions in an electrochemical biosensor has the advantage of broadening the target substrates as well as their selectivity using enzymes in different nanocompartments (=compartmentalization). The polymeric vesicles allow the loading of enzymes or artificial enzymes, offering active center retention and long-term enzyme stability. Firstly, the inclusion of spatially separated polymeric active nanocompartments into the matrix on the electrode surface is used to monitor a simple enzyme reaction. However, the study's accomplishment lies in its successful ability to monitor an enzyme cascade reaction, one producing and the other consuming hydrogen peroxide, hosting natural and artificial enzymes. This proof-of-concept generates an important contribution to the design of analytical platforms capable of detecting complex environments or even monitoring communication between cell-like structures, extremely useful for fields such as synthetic biology, biomimetics and advanced biosensors.
Ayudas:	Agencia Estatal de Investigación PID2021-126253OB-C21
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Composite ; Enzymes ; Hemin chloride ; Nanocompartments ; Nanozymes ; Polymersomes
Publicado en:	Microchemical Journal, Vol. 204 (September 2024) , art. 111071, ISSN 0026-265X

DOI: 10.1016/j.microc.2024.111071

10 p, 3.9 MB

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