The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets
Goršak, Tanja (Jožef Stefan International Postgraduate School)
Jovičić, Eva Jarc (Jožef Stefan Institute. Department of Molecular and Biomedical Sciences)
Tratnjek, Larisa (University of Ljubljana. Institute of Cell Biology)
Križaj, Igor (Jožef Stefan Institute. Department of Molecular and Biomedical Sciences)
Sepulveda, Borja (Instituto de Microelectrónica de Barcelona)
Nogués, Josep (Institut Català de Nanociència i Nanotecnologia)
Kreft, Mateja E. (University of Ljubljana. Institute of Cell Biology)
Petan, Toni (Jožef Stefan Institute. Department of Molecular and Biomedical Sciences)
Kralj, Slavko (Jožef Stefan Institute. Department for Materials Synthesis)
Makovec, Darko (Jožef Stefan International Postgraduate School)

Fecha:	2024
Resumen:	Magneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of magnetic nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, nanoparticles in cells usually agglomerate, thus greatly augmenting the delivered force compared to single nanoparticles. Here, we demonstrate that MMA also decreases the cell viability, with the MMA mediated by individual, non-interacting nanoparticles. The effect was demonstrated with ferrimagnetic (i. e. , permanently magnetic) barium-hexaferrite nanoplatelets (NPLs, ∼50 nm wide and 3 nm thick) with a unique, perpendicular orientation of the magnetization. Two cancer-cell lines (MDA-MB-231 and HeLa) are exposed to the NPLs in-vitro under different cell-culture conditions and actuated with a uniaxial AMF. TEM analyses show that only a small number of NPLs internalize in the cells, always situated in membrane-enclosed compartments of the endosomal-lysosomal system. Most compartments contain 1-2 NPLs and only seldom are the NPLs found in small groups, but never in close contact or mutually oriented. Even at low concentrations, the single NPLs reduce the cell viability when actuated with AMFs, which is further increased when the cells are in starvation conditions. These results pave the way for more efficient in-vivo MMA at very low particle concentrations.
Ayudas:	Agencia Estatal de Investigación PID2019-106229RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00651 Agencia Estatal de Investigación CEX2021-001214-S
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Magneto-mechanical actuation ; Hexaferrite nanoplatelets ; Cancer ; Cell viability ; Colloidal stability
Publicado en:	Journal of colloid and interface science, Vol. 657 (March 2024) , p. 778-787, ISSN 1095-7103

DOI: 10.1016/j.jcis.2023.12.019

10 p, 4.7 MB

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