Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
Rivaya, Belén (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Le Roy, Chloé (University of Bordeaux)
Jordana-Lluch, Elena (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Fernandez Rivas, Gema (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Casañ, Cristina (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
González Soler, Victoria (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Wang-Wang, Jun Hao (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Bébéar, Cécile (University of Bordeaux)
Matas, Lurdes (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pereyre, Sabine (University of Bordeaux)

Fecha:	2022
Resumen:	Macrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus ® MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the parC gene. Regarding MG detection, Allplex and Resistance Plus ® showed an overall agreement of 87%, but this value rose to 95. 4% if we compare them for MLr detection. Prevalence of MLr was 23. 1% in Badalona, but this rate increased to 73. 7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient's management applying resistance-guided treatment strategies.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Mycoplasma genitalium ; Macrolide ; Fluoroquinolone ; Resistance ; Spain
Publicado en:	Antibiotics, Vol. 11, Núm. 4 (April 2022) , ISSN 2079-6382

DOI: 10.3390/antibiotics11040485
PMID: 35453236

10 p, 749.8 KB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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