Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii
Severinsen, Manja Mølgaard (BOKU University. Department of Biotechnology)
Bachleitner, Simone (BOKU University. Department of Biotechnology)
Modenese, Viola (University of Milan. Department of Food, Environmental and Nutritional Sciences)
Ata, Özge (Austrian Centre of Industrial Biotechnology (Vienna))
Mattanovich, Diethard (BOKU University. Department of Biotechnology)

Date:	2024
Abstract:	Background: Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single-carbon feedstocks, derived from CO2, represent promising substrates for biotechnological applications. Especially, methanol is gaining prominence for bio-production of commodity chemicals. Results: In this study, we show the potential of Komagataella phaffii as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from Aspergillus terreus (cadA, mttA and mfsA) alongside fine-tuning of the mfsA gene expression, led to promising initial itaconic acid titers of 28 g·L-1 after 5 days of fed-batch cultivation. Through the combined efforts of process optimization and strain engineering strategies, we further boosted the itaconic acid production reaching titers of 55 g·L-1 after less than 5 days of methanol feed, while increasing the product yield on methanol from 0. 06 g·g-1 to 0. 24 g·g-1. Conclusion: Our results highlight the potential of K. phaffii as a methanol-based platform organism for sustainable biochemical production.
Grants:	European Commission 101000441 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1462
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Biotechnology for Biofuels and Bioproducts, Vol. 17 (July 2024) , art. 98, ISSN 2731-3654

DOI: 10.1186/s13068-024-02541-1
PMID: 39010147

14 p, 1.7 MB

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