Screening Traditional Foods for the Prevention of Enterotoxigenic Escherichia coli K88ac (F4ac) Attachment to IPEC-J2 Cells
Zhu, Yanan (Shaanxi University of Science and Technology)
Shao, Changyan (Shaanxi University of Science and Technology)
Martín Orúe, Susana M (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Date:	2024
Abstract:	Enterotoxigenic Escherichia coli (ETEC) is the major diarrhoea-causing pathogen world-wide. Fimbria-receptor recognition is the primary step when attachment of ETEC to the intestine occurs. This study aims to evaluate the potential of some traditional foods, particularly those rich in β-glucans, as analogues for fimbriae or receptors in reducing ETEC colonisation. The adhesion test (AT) demonstrated that aqueous extracts of highland barley (EHB), black rice (EBR) and little millet (ELT) at concentrations of 2% and 1% could attach to more ETEC K88ac (p < 0. 001), as well as aqueous extracts of shiitake (EST) (p < 0. 01). The competition test (CT) revealed that EHB and EST significantly prevented ETEC K88ac from adhering to intestinal epithelial cells (IPEC-J2) at 2% (p < 0. 01) and 1% (p < 0. 05). In the Exclusion Test (ET) and the displacement test (DT), the food samples were unable to impair ETEC colonisation in terms of blocking receptors or removing attached pathogens. These results demonstrate how some traditional foods such as highland barley and shiitake contain bioactive compounds that interfere with the attachment of ETEC to the intestinal epithelium, and their potential in the prevention and treatment of ETEC diarrhoea.
Grants:	Ministerio de Ciencia e Innovación AGL2009-07328
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Adhesion ; ETEC K88ac ; Highland barley ; Intestine ; Shiitake ; SDG 3 - Good Health and Well-being
Published in:	Foods, Vol. 13 Núm. 6 (2024) , p. 952, ISSN 2304-8158

DOI: 10.3390/foods13060952
PMID: 38540942

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