Effect of follicle size on hormonal status of follicular fluid, oocyte ATP content, and in vitro embryo production in prepubertal sheep
Contreras-Solís, Ignacio
Catalá, Maria-Gracia (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Soto-Heras, Sandra (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Roura, Montserrat (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Paramio, María Teresa (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Izquierdo Tugas, Ma. Dolors (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Date:	2021
Abstract:	The aim of this study was to assess the effect of follicular size on estradiol (E2) and progesterone (P4) levels in intrafollicular fluid, ATP content in oocytes, and the embryo development rate in prepubertal sheep. Slaughterhouse ovaries were dissected to recover the follicles, which were classified according to the follicle diameter as <3 mm (n = 20) and ≥3 mm (n = 17). Then, follicular fluid was obtained and analyzed by radioimmunoassay to determine the E2 and P4 concentrations. Another group of ovaries was used to recover cumulus-oocyte complexes according to follicle size. In vitro maturation (IVM), in vitro fertilization (IVF), and embryo culture were performed using standard procedures, and ATP level was assessed at 0 and 24 h of IVM. Intrafollicular concentrations of E2 and P4 and E2:P4 ratio were higher in ≥3 mm (18. 7 ± 5. 9 ng/mL, 7. 8 ± 1. 2 ng/mL, and 3. 6 ± 1. 3, respectively) than <3 mm (1. 8 ± 0. 4 ng/mL, 2. 6 ± 0. 3 ng/mL and 0. 9 ± 0. 3, respectively) follicles. The rate of ATP increased during IVM and was higher in oocytes from ≥3 mm than <3 mm (22. 4 ± 0. 7 and 8. 6 ± 2. 2-fold change; respectively) follicles. After IVF, the blastocyst development was higher in oocytes recovered from ≥3 mm (11. 1 ± 0. 9%) than from <3 mm (6. 5 ± 0. 7%) follicles. These results indicate an improvement in the competence and development of oocytes from ≥3 mm follicles with a higher E2:P4 ratio. Thus, this ratio could be used as reference to design IVM medium and to enhance the in vitro embryo production in lambs.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Prepubertal Ewe ; Follicular fluid ; Steroids ; Oocyte ; ATP ; In vitro embryo production
Published in:	Domestic Animal Endocrinology, Vol. 75 (April 2021) , p. 106582, ISSN 1879-0054

DOI: 10.1016/j.domaniend.2020.106582

28 p, 1.3 MB

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