Chromosomal positioning in spermatogenic cells is influenced by chromosomal factors associated with gene activity, bouquet formation and meiotic sex chromosome inactivation
Solé Canal, Mireia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Blanco, Joan (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Gil, Debora (Universitat Autònoma de Barcelona. Departament de Ciències de la Computació)
Valero, O. (Universitat Autònoma de Barcelona)
Pascual, Á (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Cárdenas, B. (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Fonseka, Gothami (Cytocell (Cambridge, Regne Unit))
Anton, Ester (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Frodsham, Richard (Cytocell (Cambridge, Regne Unit))
Vidal, Francesca (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Sarrate Navas, Zaida (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Fecha:	2021
Descripción:	13 pàg.
Resumen:	Chromosome territoriality is not random along the cell cycle and it is mainly governed by intrinsic chromosome factors and gene expression patterns. Conversely, very few studies have explored the factors that determine chromosome territoriality and its influencing factors during meiosis. In this study, we analysed chromosome positioning in murine spermatogenic cells using three-dimensionally fluorescence in situ hybridization-based methodology, which allows the analysis of the entire karyotype. The main objective of the study was to decipher chromosome positioning in a radial axis (all analysed germ-cell nuclei) and longitudinal axis (only spermatozoa) and to identify the chromosomal factors that regulate such an arrangement. Results demonstrated that the radial positioning of chromosomes during spermatogenesis was cell-type specific and influenced by chromosomal factors associated to gene activity. Chromosomes with specific features that enhance transcription (high GC content, high gene density and high numbers of predicted expressed genes) were preferentially observed in the inner part of the nucleus in virtually all cell types. Moreover, the position of the sex chromosomes was influenced by their transcriptional status, from the periphery of the nucleus when its activity was repressed (pachytene) to a more internal position when it is partially activated (spermatid). At pachytene, chromosome positioning was also influenced by chromosome size due to the bouquet formation. Longitudinal chromosome positioning in the sperm nucleus was not random either, suggesting the importance of ordered longitudinal positioning for the release and activation of the paternal genome after fertilisation.
Ayudas:	Ministerio de Economía y Competitividad DPI201565286-R Ministerio de Economía y Competitividad SAF2016-77165-P Agencia Estatal de Investigación RTI2018-095209 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1624
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Chromosome territories ; FISH ; Spermatogenesis ; Three-dimensional analysis
Publicado en:	Chromosoma, Vol. 130 Núm. 2-3 (2021) , p. 163-175, ISSN 1432-0886

DOI: 10.1007/s00412-021-00761-0
PMID: 34231035

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