The nop gene from Phanerochaete chrysosporium encodes a peroxidase with novel structural features

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(Pontificia Universidad Católica de Chile)
Perez-Acle, T

(Pontificia Universidad Católica de Chile)
Cullen, Dan (USDA Forest Products Laboratory)
Vicuña, Rafael (Pontificia Universidad Católica de Chile)

Fecha:	2005
Resumen:	Inspection of the genome of the ligninolytic basidiomycete Phanerochaete chrysosporium revealed an unusual peroxidase_like sequence. The corresponding full length cDNA was sequenced and an archetypal secretion signal predicted. The deduced mature protein (NoP, novel peroxidase) contains 295 aa residues and is therefore considerably shorter than other Class II (fungal) peroxidases, such as lignin peroxidases and manganese peroxidases. Comparative modeling of NoP was conducted using the crystal structures of Coprinus cinereus and Arthromyces ramosus peroxidases as templates. The model was validated by molecular dynamics and showed several novel structural features. In particular, NoP has only three disulfide bridges and tryptophan replaces the distal phenylalanine within the heme pocket.
Nota:	Altres ajuts: Millenium Institute for Fundamental and Applied Biology (1030495); U.S. Department of Energy (DE-FG02-87ER13712)
Derechos:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió sotmesa a revisió
Materia:	Modeling ; Molecular dynamics ; Oxidoreductase ; Peroxidase ; Phanerochaete chrysosporium
Publicado en:	Biophysical Chemistry, Vol. 116, Núm. 2 (July 2005) , p. 167-173, ISSN 1873-4200

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